T3913
Tris-Borate-EDTA buffer
5× concentrate, powder blend
Recommended Products
Quality Level
form
powder blend
technique(s)
electrophoresis: suitable
impurities
DNase, RNase and NICKase, none detected
pH
8.1-8.5 (25 °C, 5 ×)
solubility
water: 85.1 g/L, clear, colorless
suitability
suitable for gel electrophoresis (after dilution to working concentration)
application(s)
diagnostic assay manufacturing
SMILES string
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key
OSBLTNPMIGYQGY-UHFFFAOYSA-N
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Related Categories
Application
It may be employed for the following studies:
- As buffer system for the evaluation of molecular weights of glycoproteins of ovalbumin, avidin and fetuin by sodium dodecyl sulfate-pore gradient electrophoresis.
- For the hemoglobin electrophoresis in starch gel.
- Preparation of buffer concentration gradient gel for the rapid determination of DNA sequence.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Packaging
Preparation Note
Reconstitution
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P2 (EN 143) respirator cartridges
Certificates of Analysis (COA)
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Protocols
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
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