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T3913

Sigma-Aldrich

Tris-Borate-EDTA buffer

5× concentrate, powder blend

Synonym(s):
TBE buffer
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

form

powder blend

application(s)

electrophoresis: suitable

impurities

DNase, RNase and NICKase, none detected

pH

8.1-8.5 (25 °C, 5 ×)

solubility

water: 85.1 g/L, clear, colorless

suitability

suitable for gel electrophoresis (after dilution to working concentration)

Featured Industry

Diagnostic Assay Manufacturing

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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Application

Tris-Borate-EDTA buffer is suitable for use in gel electrophoresis.
It may be employed for the following studies:
  • As buffer system for the evaluation of molecular weights of glycoproteins of ovalbumin, avidin and fetuin by sodium dodecyl sulfate-pore gradient electrophoresis.
  • For the hemoglobin electrophoresis in starch gel.
  • Preparation of buffer concentration gradient gel for the rapid determination of DNA sequence.
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

Packaged in pouches

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Reconstitution

Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water. A suitable container must be supplied.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

THE AMINO ACID COMPOSITION OF HEMOGLOBIN. V. THE PREPARATION OF PURIFIED HEMOGLOBIN FRACTIONS BY CHROMATOGRAPHY ON CELLULOSE EXCHANGERS AND THEIR IDENTIFICATION BY STARCH GEL ELECTROPHORESIS USING TRIS-BORATE-EDTA BUFFER.
A I CHERNOFF et al.
Blood, 25, 646-661 (1965-05-01)
M D Biggin et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(13), 3963-3965 (1983-07-01)
Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. We have developed a rapid way to pour a buffer concentration gradient gel that, by altering the vertical band separation...
Glycoprotein molecular-weight estimation using sodium dodecyl sulfate-pore gradient electrophoresis: comparison of tris-glycine and tris-borate-EDTA buffer systems.
J F Poduslo
Analytical biochemistry, 114(1), 131-139 (1981-06-01)
Xiang Li et al.
Nucleic acids research, 47(10), 5074-5085 (2019-06-05)
In microorganisms, a number of metalloproteins including PerR are found to regulate gene expression in response to environmental reactive oxygen species (ROS) changes. However, discovery of similar regulatory mechanisms remains elusive within mammalian cells. As an antioxidant metalloenzyme that maintains...
Josep Balart et al.
Radiation oncology (London, England), 6, 6-6 (2011-01-18)
Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mechanisms...

Protocols

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

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