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T4026

Sigma-Aldrich

Monoclonal Anti-β-Tubulin antibody produced in mouse

enhanced validation

clone TUB 2.1, ascites fluid

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Synonym(s):
Monoclonal Anti-β-Tubulin
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

TUB 2.1, monoclonal

mol wt

antigen 55 kDa

contains

15 mM sodium azide

species reactivity

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

enhanced validation

independent ( Antibodies)
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:200 using cultured chicken fibroblasts
western blot: 1:100 using human or chicken fibroblast cell extract

isotype

IgG1

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. β-Tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)).
Monoclonal Anti-b-Tubulin (mouse IgG1 isotype) is derived from the hybridoma TUB 2.1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified rat brain tubulin.

Specificity

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Immunogen

tubulin from rat brain.

Application

Monoclonal Anti β-Tubulin antibody produced in mouse has been used in following studies:
  • Fluorescence-activated cell sorting (FACS)
  • Immunoblotting
  • Immunofluorescence Analysis

Biochem/physiol Actions

Mutation in the β-tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β- 1 tubulin also known as Class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

Physical form

Supplied as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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