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T4069

JC-1

≥97% purity, solid

Synonym(s):

5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide, 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide, CBIC2(3)

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5 MG

$683.00

$683.00

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About This Item

Empirical Formula (Hill Notation):
C25H27Cl4IN4
CAS Number:
3520-43-2
Molecular Weight:
652.23
UNSPSC Code:
12171500
PubChem Substance ID:
24900193
NACRES:
NA.47
MDL number:
MFCD00188475

Key Documents

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Product Name

JC-1, powder or solid (Crystals)

SMILES string

[I-].CCN1C(=C\C=C\c2n(CC)c3cc(Cl)c(Cl)cc3[n+]2CC)/N(CC)c4cc(Cl)c(Cl)cc14

InChI

1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1

InChI key

FYNNIUVBDKICAX-UHFFFAOYSA-M

assay

≥97%

form

powder or solid (Crystals)

technique(s)

titration: suitable

color

faint red to very dark red

ε (extinction coefficient)

≥175000 at 511-517 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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This Item
SML3593PHL8916650636
JC-1 powder or solid (Crystals)

Sigma-Aldrich

T4069

JC-1

Hexachlorophene ≥95% (HPLC)

Sigma-Aldrich

SML3593

Hexachlorophene

I3,II8-Biapigenin phyproof® Reference Substance

PHL89166

I3,II8-Biapigenin

Paraquat dichloride-(rings-d8) hydrate PESTANAL®, analytical standard

Supelco

50636

Paraquat dichloride-(rings-d8) hydrate

technique(s)

titration: suitable

technique(s)

-

technique(s)

-

technique(s)

-

color

faint red to very dark red

color

white to beige

color

-

color

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

application(s)

agriculture

form

powder or solid (Crystals)

form

powder

form

solid

form

-

assay

≥97%

assay

≥95% (HPLC)

assay

≥98.0% (HPLC)

assay

≥98.0% (HPLC)

storage temp.

room temp

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

The cyanine dye JC-1 has been used to determine mitochondrial transmembrane potential.
A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ5582
MKCX9305
MKCX2204
MKCW5291
MKCT7568

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Junctophilin 3 expresses in pancreatic beta cells and is required for glucose-stimulated insulin secretion
Li L
Cell Death & Disease (2016)
Shumin Qin et al.
Frontiers in pharmacology, 10, 894-894 (2019-09-03)
Alcohol consumption affects gastric mucosa by multiple and complex mechanisms depending either by direct contact of ethanol or by indirect biological damage induced by its metabolite acetaldehyde. The present study aims at further investigating the mechanism of ethanol-induced gastric mucosa
Qiong Wang et al.
Acta biomaterialia, 94, 469-481 (2019-05-30)
Multi-drug resistance (MDR) of tumor is a major cause of chemotherapy failure. In this study, a pH-sensitive graft copolymer, poly(β-amino ester)-g-β-cyclodextrin (PBAE-g-β-CD), was synthesized via Michael addition polymerization and was employed to co-deliver doxorubicin (DOX), a chemotherapy agent, and adjudin
Sulfated polysaccharide-protein complex sensitizes doxorubicin-induced apoptosis of breast cancer cells in vitro and in vivo
Jie Wang
Experimental and Therapeutic Medicine (2016)
JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry
A Perelman
Cell Death & Disease (2012)
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Questions

  1. Mitochondria Staining Kit (CS0390)에 들어있는 1MG-JC-1 powder와 다른 제품인가요? 동일 농도로 DMSO이용해 녹였더니 Stock 색상이 다릅니다.

    1 answer

    1. Yes, the T4069, JC-1 product is also supplied in the Mitochndria Stain kit, CS0390. Both are prepared at 1 mg/mL in DMSO resulting in a red solution. Slight variations in color may be considered normal and the result of lot-to-lot variability. Marked differences in color should be discussed with Technical Service.

      Should this be the case, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section and respond with all the required information so that a member of the Technical Service team can reach out to assist further. Thank you.

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