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T4069

Sigma-Aldrich

JC-1

solid

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Synonym(s):
5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide, 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide, CBIC2(3)
Empirical Formula (Hill Notation):
C25H27Cl4IN4
CAS Number:
3520-43-2
Molecular Weight:
652.23
MDL number:
MFCD00188475
PubChem Substance ID:
24900193
NACRES:
NA.47

Assay

≥97%

Quality Level

100

form

solid

ε (extinction coefficient)

≥175000 at 511-517 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[I-].CCN1C(=C\C=C\c2n(CC)c3cc(Cl)c(Cl)cc3[n+]2CC)/N(CC)c4cc(Cl)c(Cl)cc14

InChI

1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1

InChI key

FYNNIUVBDKICAX-UHFFFAOYSA-M

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This Item
SML3593693324PHL89166
JC-1 solid

Sigma-Aldrich

T4069

JC-1

Hexachlorophene ≥98% (HPLC)

Sigma-Aldrich

SML3593

Hexachlorophene

(S)-[(RuCl(H8-BINAP))2(μ-Cl)3][NH2Me2]

Sigma-Aldrich

693324

(S)-[(RuCl(H8-BINAP))2(μ-Cl)3][NH2Me2]

I3,II8-Biapigenin phyproof® Reference Substance

PHL89166

I3,II8-Biapigenin

form

solid

form

powder

form

powder

form

solid

ε (extinction coefficient)

≥175000 at 511-517 nm in methanol

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

application(s)

-

storage temp.

room temp

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

The cyanine dye JC-1 has been used to determine mitochondrial transmembrane potential.
A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Xianbin Cao et al.
Cellular signalling, 62, 109337-109337 (2019-06-08)
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Sulfated polysaccharide-protein complex sensitizes doxorubicin-induced apoptosis of breast cancer cells in vitro and in vivo
Jie Wang
Experimental and Therapeutic Medicine (2016)
JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry
A Perelman
Cell Death & Disease (2012)
Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis
Chunyan Xue
Oncology Letters (2016)
Sudhir Pandey et al.
American journal of physiology. Cell physiology, 317(2), C235-C243 (2019-05-23)
Doxorubicin (DOX) is an anthracycline antibiotic commonly employed for the treatment of various cancers. However, its therapeutic uses are hampered by side effects associated with cumulative doses during the course of treatment. Whereas deregulation of autophagy in the myocardium has
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