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T4184

Sigma-Aldrich

Thrombopoietin from mouse

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture, >97% (SDS-PAGE)

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Synonym(s):
TPO
CAS Number:
MDL number:
NACRES:
NA.32

biological source

mouse

Quality Level

recombinant

expressed in NSO cells

Assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

2.1 ng/mL EC50

mol wt

~75 kDa by SDS-PAGE (as a result of glycosylation)
predicted mol wt 35 kDa

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Thpo(21832)

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TRAIL human

recombinant

expressed in NSO cells

recombinant

expressed in NSO cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in NSO cells

assay

>97% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

>97% (SDS-PAGE)

assay

>97% (SDS-PAGE)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

potency

2.1 ng/mL EC50

potency

1.030 ng/mL

potency

-

potency

-

mol wt

~75 kDa by SDS-PAGE (as a result of glycosylation), predicted mol wt 35 kDa

mol wt

~36 kDa by SDS-PAGE, predicted mol wt 18.6 kDa

mol wt

~38 kDa by SDS-PAGE

mol wt

24 kDa by SDS-PAGE, predicted mol wt ~21 kDa

Application

Thrombopoietin from mouse can be used in a genetic screening test for examining the role of somatic activating mutations in EPOR, MPL, or GCSFR genes in activating myeloproliferative disorders (MPD) in JAK2V617F-negative MPD patients.

Biochem/physiol Actions

Thrombopoeitin (TPO), also known as the ligand for the c-mpl proto-oncogene (Mpl-L) and as megakaryocyte colony stimulating factor (Meg-CSF), is a primary regulatory factor for megakaryocytopoiesis and thrombopoiesis. Produced mainly by hepatocytes but also by kidney cells, TPO is a humoral glycoprotein that stimulates growth and maturation of megakaryocytes and megakaryocytic colonies from bone marrow cultures. TPO binds and activates an 68-78 kDa glycoprotein receptor belonging to the GH family of cytokine receptors, a family that includes receptors to growth hormone (GH), erythropoietin (EPO) and prolactin (PRL). Like GH and EPO, TPO may bind to its receptor at two distinct sites, initiating receptor dimerization and activation. Analysis of mRNA indicates also the existence of a novel truncated and potentially soluble form of TPO receptor. The viral oncogene v-mlp of the myeloproliferative leukemia virus (MPLV) contains the gene sequence for the entire cytoplasmic and transmembrane domains and a portion of the extracellular domain of c-mlp (TPO receptor). TPO receptors are found on megakaryocytes and their precursors and on platelets. The mature form of TPO is a highly conserved glycoprotein, showing approximately 70% sequence homology among various mammals. Human TPO contains 332 amino acids (mouse 335) including two internal disulfide bonds and 6 N-linked glycosylation sites (mouse 7). The N-terminal 153 amino acid region of TPO shows structural and amino acid sequence homology to erythropoietin and a pair of arginines (potential cleavage site) separating it from the highly glycosylated carboxyterminal domain.
Thrombopoietin from mouse is an amino acid peptide that plays a pivotal role in megakaryocyte proliferation and differentiation. It also serves as a growth factor for liver endothelial cells in mouse.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin

Analysis Note

The biological activity was measured in a cell proliferation assay using MO7e cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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P Rojnuckarin et al.
Blood, 94(4), 1273-1282 (1999-08-10)
Thrombopoietin (TPO) plays a critical role in megakaryocyte proliferation and differentiation. Using various cultured cell lines, several recent studies have implicated the mitogen-activated protein kinase (MAPK) pathway in megakaryocyte differentiation. In the study reported here, we examined the role played
J E Cardier et al.
Blood, 91(3), 923-929 (1998-02-03)
Present data suggest that the primary site of thrombopoietin (TPO) mRNA is the liver. Previously, we reported that specific murine liver endothelial cells (LEC-1) located in the hepatic sinusoids support in vitro megakaryocytopoiesis from murine hematopoietic stem cells suggesting that
Shun-Mei Lu et al.
Brain, behavior, and immunity, 44, 221-234 (2014-12-03)
Neuro-inflammation plays a key role in the occurrence and development of postoperative cognitive dysfunction (POCD). Although S100A8 and Toll-like receptor 4 (TLR4) have been increasingly recognized to contribute to neuro-inflammation, little is known about the interaction between S100A8 and TLR4/MyD88
Yana Pikman et al.
PLoS medicine, 3(7), e270-e270 (2006-07-13)
The JAK2V617F allele has recently been identified in patients with polycythemia vera (PV), essential thrombocytosis (ET), and myelofibrosis with myeloid metaplasia (MF). Subsequent analysis has shown that constitutive activation of the JAK-STAT signal transduction pathway is an important pathogenetic event
L M Privette Vinnedge et al.
Oncogene, 34(18), 2325-2336 (2014-06-24)
Disease progression and recurrence are major barriers to survival for breast cancer patients. Understanding the etiology of recurrent or metastatic breast cancer and underlying mechanisms is critical for the development of new treatments and improved survival. Here, we report that

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