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T4415

Sigma-Aldrich

Tris-Borate-EDTA buffer

BioReagent, suitable for electrophoresis, 10× concentrate

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Synonym(s):
TBE buffer
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

product line

BioReagent

form

solution

impurities

DNase and RNase, none detected

suitability

suitable for electrophoresis

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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1 of 4

This Item
T7527T39138820-OP
Tris-Borate-EDTA buffer BioReagent, suitable for electrophoresis, 10× concentrate

Sigma-Aldrich

T4415

Tris-Borate-EDTA buffer

Essential+ Grade
Tris-Borate-EDTA buffer BioReagent, for molecular biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Sigma-Aldrich

T7527

Tris-Borate-EDTA buffer

Essential+ Grade
Tris-Borate-EDTA buffer 5× concentrate, powder blend

Sigma-Aldrich

T3913

Tris-Borate-EDTA buffer

-
Millipore

Millipore

8820-OP

TBE Buffer

-
form

solution

form

powder blend

form

powder blend

form

liquid

impurities

DNase and RNase, none detected

impurities

DNase and RNase, none detected

impurities

DNase, RNase and NICKase, none detected

impurities

-

suitability

suitable for electrophoresis

suitability

suitable for electrophoresis

suitability

-

suitability

-

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

100

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).

Application

Ready for use in gel electrophoresis after dilution to working concentrations.
Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

The 4L, 10L and 20L sizes are supplied in dispenser with a spigot.

Preparation Note

Prepared with 18 megohm water
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Other Notes

TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
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Millipore

Millipore

8820-OP

TBE Buffer

Characterization of a Clavibacter michiganensis subsp. michiganensis population in Israel.
Kleitman, F., Barash, I., Burger, A., Iraki, N.,
European Journal of Plant Pathology, 121, 463-475 (2008)
M Wu et al.
BioTechniques, 24(4), 676-678 (1998-06-13)
A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel-strength agarose
L Orbán et al.
Electrophoresis, 12(4), 233-240 (1991-04-01)
DNA fragments up to 9 kb in size were stacked and separated by polyacrylamide gel electrophoresis, and those up to 50 kb in size by agarose gel electrophoresis, using a discontinuous buffer system. Polyacrylamide gels at pH 8.9, 2 degrees
Josep Balart et al.
Radiation oncology (London, England), 6, 6-6 (2011-01-18)
Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mechanisms
Yaroslav A Kainov et al.
Nature communications, 11(1), 361-361 (2020-01-19)
Eukaryotic gene expression relies on extensive crosstalk between transcription and RNA processing. Changes in this composite regulation network may provide an important means for shaping cell type-specific transcriptomes. Here we show that the RNA-associated protein Srrt/Ars2 sustains embryonic stem cell

Protocols

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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