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MilliporeSigma

T4528

Sigma-Aldrich

Thioglucosidase from Sinapis alba (white mustard) seed

≥100 units/g solid

Synonym(s):

Glucosinolase, Myrosinase, Thioglucoside glucohydrolase

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25 UNITS
$137.00
100 UNITS
$502.00
200 UNITS
$880.00

$137.00


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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

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biological source

plant seeds (Sinapis alba)

Quality Level

form

solid

specific activity

≥100 units/g solid

storage temp.

−20°C

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This Item
A9228G4511D8144
biological source

plant seeds (Sinapis alba)

biological source

-

biological source

-

biological source

Penicillium sp.

specific activity

≥100 units/g solid

specific activity

≥40,000 units/g solid

specific activity

10-50 units/mg solid

specific activity

400-800 units/mg protein

form

solid

form

lyophilized solid

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

General description

Thioglucosidase, also called as myrosinase, is present in the myrosin cells that do not contain glucosinolates.[1] This enzyme is obtained from several plant sources, such as Lepidium sativum, L. Sinapis alba and Brassica napus.[2]

Application

Thioglucosidase from Sinapis alba (white mustard) seeds has been used as a standard to quantify myrosinase activity[3] and in column glucosinolate analysis of plant samples.[4]
Thioglucosidase has been used in a study to assess Brassica species screening for glucosinolate content. [5] Thioglucosidase has also been used in a study to investigate a negative regulatory role for auxin in sulphate deficiency response in Arabidopsis thaliana. [6]

Biochem/physiol Actions

Myrosinases are present in many bacteria, fungi, and edible plants, including those of the Brassicaceae (Cruciferae) family. The enzyme hydrolyzes the S-glucosidic bond of a glucosinolate substrate to form an unstable aglycone that rearranges with the loss of sulfate primarily to the isothiocyanate, though thiocyanates and nitriles are also formed. Many of the isothiocyanate products of aliphatic and aromatic glucosinolates have cancer chemopreventive properties.
Thioglucosidase research has focused mainly on the cruciferous crops due to their economic importance and cancer preventive benefits. [7]

Other Notes

One unit will produce 1.0 μmole glucose per min from sinigrin at pH 6.0 at 25 °C.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Wei Chen et al.
Frontiers in physiology, 13, 1013092-1013092 (2022-11-08)
Glycoside hydrolase family 1 (GH1) members exhibit a broad substrate spectrum and play important roles in insect-plant interactions, such as the defensive β-glucosidase and β-thioglucosidase (so-called myrosinase). However, knowledge about the expression profiling and function of glycoside hydrolase family 1
Niels Agerbirk et al.
Phytochemistry, 153, 79-93 (2018-06-11)
Glucosinolates are found in plants of the order Brassicales and hydrolyzed to different breakdown products, particularly after tissue damage. In Barbarea vulgaris R.Br. (Brassicaceae), the dominant glucosinolate in the investigated "G-type" is glucobarbarin, (S)-2-hydroxy-2-phenylethylglucosinolate. Formation of the nitrile from glucobarbarin
Teresa Oliviero et al.
Molecular nutrition & food research, 62(18), e1700837-e1700837 (2018-03-14)
Optimization of bioavailability of dietary bioactive health-beneficial compounds is as important as increasing their concentration in foods. The aim of this study is to explore the change in bioavailability of isothiocyanates (ITCs) in broccoli sprouts incorporated in protein, fiber, and
George F Antonious et al.
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes, 44(3), 311-316 (2009-03-13)
Glucosinolates (GSLs), a group of compounds found in Brassica plants, are toxic to some soil-borne plant pathogens because of the toxicity of their hydrolysis products, isothiocyanates. Other phytochemicals found in Brassica plants, such as phenols and ascorbic acid, may compliment
Patricia A Egner et al.
Cancer prevention research (Philadelphia, Pa.), 4(3), 384-395 (2011-03-05)
One of several challenges in design of clinical chemoprevention trials is the selection of the dose, formulation, and dose schedule of the intervention agent. Therefore, a cross-over clinical trial was undertaken to compare the bioavailability and tolerability of sulforaphane from

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