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T4648

Sigma-Aldrich

Thrombin from bovine plasma

lyophilized powder, 40-300 NIH units/mg protein (biuret)

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Synonym(s):
Factor IIa
CAS Number:
9002-04-4
Enzyme Commission number:
3.4.21.5 (BRENDA, IUBMB)
EC Number:
232-648-7
MDL number:
MFCD00166053
NACRES:
NA.54

biological source

bovine plasma

Quality Level

300

form

lyophilized powder

specific activity

40-300 NIH units/mg protein (biuret)

mol wt

heavy chain ~33 kDa
light chain ~5 kDa

composition

Protein, 40-60%

UniProt accession no.

P00735

shipped in

wet ice

storage temp.

−20°C

Gene Information

cow ... F2(280685)

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This Item
T8885T6634T7513
Thrombin from bovine plasma lyophilized powder, 40-300 NIH units/mg protein (biuret)

Sigma-Aldrich

T4648

Thrombin from bovine plasma

Thrombin from human plasma lyophilized powder, Suitable for routine use in the thrombin time test

Sigma-Aldrich

T8885

Thrombin from human plasma

Sigma-Aldrich

Sigma-Aldrich

T6634

Thrombin from bovine plasma

Sigma-Aldrich

Sigma-Aldrich

T7513

Thrombin from bovine plasma

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

specific activity

40-300 NIH units/mg protein (biuret)

specific activity

-

specific activity

600-2,000 NIH units/mg protein (biuret)

specific activity

≥2,000 NIH units/mg protein (E1%/280 = 19.5)

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

mol wt

-

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

UniProt accession no.

P00735

UniProt accession no.

P00734

UniProt accession no.

P00735

UniProt accession no.

P00735

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess persistent hypocoagulability in patients with septic shock.

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Unit Definition

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

Physical form

Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0

Preparation Note

Activated with bovine brain thromboplastin.

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

inhibitor

Product No.
Description
Pricing

substrate

Product No.
Description
Pricing

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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