T5050

Sigma-Aldrich

Transforming Growth Factor-β1 from porcine platelets

TGF-β1, powder, suitable for cell culture

Synonym(s):
TGF-β1
MDL number:
NACRES:
NA.77
Pricing and availability is not currently available.

biological source

Porcine (Platelets)

Quality Level

assay

≥97% (SDS-PAGE)

form

powder

potency

0.01-0.1 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

protein 25 kDa

packaging

pkg of 1 μg
pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

pig ... TGFB1(397078)

General description

The TGFB (transforming growth factor-β) gene is mapped to pig (Sus scrofa) chromosome between 6q11-q21. In mammals, TGFβ is present in three isoforms (TGFβ1, TGFβ2 and TGFβ3), of which TGFβ1 is predominant.

Application

Transforming Growth Factor-β1 from porcine platelets has been used in chondrogenic differentiation. It has also been used in immobilization process.

Biochem/physiol Actions

Transforming growth factor-β1 (TGF-β) is a multifunctional polypeptide growth factor. TGF-β mediates cell proliferation, differentiation and migration. Important cellular functions such as inflammation, extracellular matrix synthesis, epithelial restoration and developmental processes are also controlled by TGF-β. TGF-β controls the regulation of enamel mineralization in porcine. TGF-β is associated with the development of idiopathic pulmonary fibrosis (a chronic lung disease). TGF-β promotes epithelial-mesenchymal transition (EMT) and contributes to proliferation and metastasis of cancer. The TGF-β induced EMT is observed in kidney fibrosis. TGF-β is stimulates regeneration of intestinal tissue.
Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 0.05 mg bovine serum albumin per μg cytokine.

Analysis Note

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
QTL for the heritable inverted teat defect in pigs.
Jonas E, et al.
Mammalian Genome, 19(2), 127-138 (2008)
Developmental Changes of TGF-β1 and Smads Signaling Pathway in Intestinal Adaption of Weaned Pigs
Xiao K, et al.
PLoS ONE, 9(8) (2014)
Effect of Conceptus on Transforming Growth Factor (TGF) β1 mRNA Expression and Protein Concentration in the Porcine Endometrium?In Vivo and In Vitro Studies
Blitek A, et al.
Journal of Reproduction and Development, 59(6), 512-519 (2013)
TGF-β1 immobilized tri-co-polymer for articular cartilage tissue engineering.
Chou CH, et al.
Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 77(2), 338-348 (2006)
Isolation, culture and chondrogenic differentiation of canine
adipose tissue- and bone marrow-derived mesenchymal stem
cells?a comparative study
Reich CM, et al.
Veterinary Research Communications, 36, 139-148 (2012)

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