MilliporeSigma
All Photos(2)

T5076

Sigma-Aldrich

Anti-β-Tubulin Isotype III antibody, Mouse monoclonal

clone SDL.3D10, purified from hybridoma cell culture

Synonym(s):
Anti-CDCBM1, Anti-CDCBM, Anti-CFEOM3A, Anti-TUBB4, Anti-CFEOM3, Anti-beta-4, Anti-FEOM3
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SDL.3D10, monoclonal

form

buffered aqueous solution

species reactivity

human, bovine, mouse, rat, boar

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using rat brain extract

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TUBB3(10381)
mouse ... Tubb3(22152)
rat ... Tubb3(246118)

General description

Tubulin β 3 class III (TUBB3) encodes for the microtubule protein tubulin β‐3, which is majorly expressed in neurons. The gene is located on human chromosome 16q24.3.
Tubulin is the main constituent of microtubules made up of two similar polypeptides α and β, each with 50 kD (approx.) molecular weight. It has several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-β-tubulin isotype III antibody can be used in microarray and western blotting. It can also be used in immunoblotting. Mouse anti-β-tubulin isotype III antibody reacts specifically with an epitope present on the β-tubulin (isotype III) of bovine, rat, mouse, boar and human but not with tubulin present in chicken brain preparation or in a chicken fibroblasts cell line.

Immunogen

peptide corresponding to the carboxy-terminal sequence of human β-tubulin isotype III, conjugated to BSA.

Application

Monoclonal Anti-β-Tubulin Isotype III antibody produced in mouse has been used in immunolabelling and flow cytometry.
Monoclonal anti-β-tubulin isotype III antibody (diluted 1:500) can be used as a primary antibody in immunocytochemical analysis of neurons. It can also be used for the localization of isotype III of β-tubulin by several immunochemical assays like dot blot, ELISA and immunohistochemistry.

Biochem/physiol Actions

Tubulin β 3 class III (TUBB3) regulates neurogenesis and is involved in axon guidance and maintenance. Mutations in this gene is associated with hypoplasia of commissural axon (CA), congenital fibrosis of the extraocular muscles type 3 (CFEOM3) and dysgyria.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Evaluation of tubulin beta-3 as a novel senescence-associated gene in melanocytic malignant transformation
Orfanidis K, et al.
Pigment Cell & Melanoma Research, 30(2), 243-254 (2017)
Cytoskeleton in action: lissencephaly, a neuronal migration disorder
Moon HM and Wynshaw-Boris A
Wiley interdisciplinary reviews. Developmental biology, 2(2), 229-245 (2013)
Childhood-onset schizophrenia case with 2.2 Mb deletion at chromosome 3p12. 2-p12. 1 and two large chromosomal abnormalities at 16q22. 3-q24. 3 and Xq23-q28
Rudd D, et al.
Clinical case reports, 3(4), 201-207 (2015)
Two unique TUBB3 mutations cause both CFEOM3 and malformations of cortical development
Whitman MC, et al.
American Journal of Medical Genetics. Part A, 170(2), 297-305 (2016)
Péter Enyedi et al.
PloS one, 9(5), e97854-e97854 (2014-05-17)
The cytoplasmic loop between the second and third transmembrane segments is pivotal in the regulation of TRESK (TWIK-related spinal cord K+ channel, K2P18.1, KCNK18). Calcineurin binds to this region and activates the channel by dephosphorylation in response to the calcium

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service