T5168

Monoclonal Anti-α-Tubulin antibody produced in mouse

ascites fluid, clone B-5-1-2

• Synonym(s): Alpha Tubulin Antibody Sigma, Anti Tubulin Sigma, Tubulin Antibody Sigma, Tubulin Antibody Sigma - Monoclonal Anti-α-Tubulin antibody produced in mouse
• MDL number: MFCD00145891
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: B-5-1-2, monoclonal
• mol wt: antigen ~50 kDa
• contains: 15 mM sodium azide
• species reactivity: mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat
• enhanced validation: independent ( Antibodies); Learn more about Antibody Enhanced Validation
• technique(s): indirect immunofluorescence: 1:2,000 using cultured human or chicken fibroblasts; radioimmunoassay: suitable; western blot: 1:4,000 using human fibroblast cell extract
• isotype: IgG1
• UniProt accession no.: P68366
• application(s): research pathology
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... TUBA4A(7277) ; mouse ... Tuba1a(22142) ; rat ... Tuba1a(64158)

Description

General description

Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the B-5-1-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin is the major building block of microtubules.

The TUBA4A (tubulin α 4a), is mapped to human chromosome 2q35. The gene codes for a member of the a -tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. The α-subunit of tubulin has molecular weight of 50,000.

Specificity

Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.

Immunogen

Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Application

Monoclonal Anti-α-Tubulin antibody produced in mouse has been used:

• In immunofluorescence Analysis
• In western blotting/ Immunoblotting
• For immunolabelling cells in electron microscopy

Biochem/physiol Actions

Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).
α-Tubulin is a key regulator of cytoskeletal proteins. It mediates cellular developmental stages such as proliferation, migration, signalling and also maintains the shape of the cell. α-Tubulin controls trafficking, signaling and cellular tensegrity mediated by microtubules. The encoded protein is associated with the development and progression of cancer. α-Tubulin acetylation potentiates the metastatic property of breast cancer. Mutation in TUBA4A is associated with the development of various types of cancers, such as oral cancer, breast cancer, rectal cancer, lung cancer and prostate cancer. In addition, variation in the TUBA4A leads to sporadic amyotrophic lateral sclerosis (ALS).
Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and the cytoskeleton.

Tubulin α mediates the formation of cytoskeletal protein polymers. It is necessary for cell cycle and controls cellular movement and signaling. Tubulin α is known to maintain the shape of the cells. It is an active part of oncogenesis and progression of different types of cancer. Posttranslationally modified Tubulin α governs microtubule functions including cell trafficking and cellular tensegrity.

Physical form

Liquid

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable