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T5168

Sigma-Aldrich

Monoclonal Anti-α-Tubulin antibody produced in mouse

enhanced validation

clone B-5-1-2, ascites fluid

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Synonym(s):
Alpha Tubulin Antibody Sigma, Anti Tubulin Sigma, Tubulin Antibody Sigma, Tubulin Antibody Sigma - Monoclonal Anti-α-Tubulin antibody produced in mouse
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

B-5-1-2, monoclonal

mol wt

antigen ~50 kDa

contains

15 mM sodium azide

species reactivity

mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat

enhanced validation

independent ( Antibodies)
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:2,000 using cultured human or chicken fibroblasts
radioimmunoassay: suitable
western blot: 1:4,000 using human fibroblast cell extract

isotype

IgG1

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

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This Item
T9026T5293T6199
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

clone

B-5-1-2, monoclonal

clone

DM1A, monoclonal

clone

2-28-33, monoclonal

clone

DM1A, monoclonal

mol wt

antigen ~50 kDa

mol wt

antigen ~50 kDa

mol wt

-

mol wt

antigen ~50 kDa

species reactivity

mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat

species reactivity

yeast, mouse, amphibian, human, rat, chicken, fungi, bovine

species reactivity

Ciona intestinalis, rat, chicken, Caenorhabditis elegans, Strongylocentrotus purpuratus, bovine, human

species reactivity

yeast, mouse, amphibian, human, rat, chicken, fungi, bovine

General description

Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the B-5-1-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin is the major building block of microtubules.
The TUBA4A (tubulin α 4a), is mapped to human chromosome 2q35. The gene codes for a member of the a -tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. The α-subunit of tubulin has molecular weight of 50,000.

Specificity

Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.

Immunogen

Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Application

Monoclonal Anti-α-Tubulin antibody produced in mouse has been used:
  • In immunofluorescence Analysis
  • In western blotting/ Immunoblotting
  • For immunolabelling cells in electron microscopy

Biochem/physiol Actions

Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).
α-Tubulin is a key regulator of cytoskeletal proteins. It mediates cellular developmental stages such as proliferation, migration, signalling and also maintains the shape of the cell. α-Tubulin controls trafficking, signaling and cellular tensegrity mediated by microtubules. The encoded protein is associated with the development and progression of cancer. α-Tubulin acetylation potentiates the metastatic property of breast cancer. Mutation in TUBA4A is associated with the development of various types of cancers, such as oral cancer, breast cancer, rectal cancer, lung cancer and prostate cancer. In addition, variation in the TUBA4A leads to sporadic amyotrophic lateral sclerosis (ALS).
Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and the cytoskeleton.
Tubulin α mediates the formation of cytoskeletal protein polymers. It is necessary for cell cycle and controls cellular movement and signaling. Tubulin α is known to maintain the shape of the cells. It is an active part of oncogenesis and progression of different types of cancer. Posttranslationally modified Tubulin α governs microtubule functions including cell trafficking and cellular tensegrity.

Physical form

Liquid

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Zhixiong Dong et al.
International journal of medical sciences, 16(8), 1132-1141 (2019-09-17)
Ribosomal biogenesis is responsible for protein synthesis in all eukaryotic cells. Perturbation of ribosomal biogenesis processes can cause dysfunctions of protein synthesis and varieties of human diseases. In this study, we examine the role of RPL15, a large ribosomal subunit
Increased expression of αTubulin is associated with poor prognosis in patients with pancreatic cancer after surgical resection
Lin C, et al.
Oncotarget, 7(37), 60657-60657 (2016)
Florian Sonthonnax et al.
Scientific reports, 9(1), 12171-12171 (2019-08-23)
Phosphoprotein (P) and matrix protein (M) cooperate to undermine the immune response to rabies virus (RABV) infections. While P is involved in the modulation of the Jak-Stat pathway through the cytoplasmic retention of interferon (IFN)-activated STAT1 (pSTAT1), M interacts with
TUBA4A gene analysis in sporadic amyotrophic lateral sclerosis: identification of novel mutations.
Pensato V, et al.
Journal of Neurology, 262(5), 1376-1378 (2015)
Centrosomes split in the presence of impaired DNA integrity during mitosis.
Hut HM
Molecular and Cellular Biology, 14(5), 1993-2004 (2003)

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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