T5425

Sigma-Aldrich

Transforming Growth Factor-β3 human

TGF-β3, recombinant, expressed in baculovirus infected Sf21 cells, powder, suitable for cell culture

Synonym(s):
hTGF-β3, TGF-β3
MDL number:
NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥97% (SDS-PAGE)

form

powder

potency

0.01-0.09 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

predicted mol wt ~25 kDa

packaging

pkg of 2 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

<0.01 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... TGFB3(7043)

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General description

The TGFβ3 (transforming Growth Factor-β3) gene is mapped to human chromosome 14q24.3.

Application

Transforming Growth Factor-β3 human has been used to study the role of TGF-β3 in healing tendon-to-bone insertion and in chondrogenic differentiation.

Biochem/physiol Actions

TGF-β (transforming growth factor-β3) isoforms are significantly involved in inducing inflammatory responses. It is associated with vascular diseases, wound healing and ameliorating effect in venous leg ulcer. TGF-β3 has an antagonistic effect on TGF-β1 and reduces the degree of collagen type I deposition and fibroblast differentiation. As a result TGF-β3 might lead to ulcer chronicity due to hindered myofibroblasts formation. TGF-β is a key player in tumor formation and progression. Increased TGF-β3 expression is observed in cancer and adipose tissue fibrosis. TGF-β3 is crucial in tissue regeneration and scarless tissue repair.
TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Tsang, M., et al.
Proc. 7th Intl. Lymphokine Workshop, 9, 607-607 (1990)
Adipose tissue fibrosis in human cancer cachexia: the role of TGFβ pathway
Alves MJ, et al.
BMC Cancer, 17: 190 (2017)
Chronic Venous Insufficiency: Transforming Growth Factor-β Isoforms and Soluble Endoglin Concentration in Different States of Wound Healing
Ligi D, et al.
International Journal of Molecular Sciences, 18(10) (2017)
Roberts, A., and Sporn, M. et al.
Peptide Growth Factors and their Receptors I, 419-472 (1991)
Chondrogenic Differentiation of Menstrual Blood-Derived Stem Cells on Nanofibrous Scaffolds
Kazemnejad S, et al.
Methods in Molecular Biology, 149-169 (2013)

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