T5530

Sigma-Aldrich

Monoclonal Anti-τ (Tau) antibody produced in mouse

clone TAU-2, ascites fluid

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

TAU-2, monoclonal

mol wt

antigen 55-62 kDa

contains

15 mM sodium azide

species reactivity

bovine, chicken, human, monkey

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
microarray: suitable
western blot: 1:1,000 using a fresh total bovine brain extract or an enriched microtubule protein preparation

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MAPT(4137)

General description

Monoclonal Anti- τ (TAU) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. τ (TAU) proteins are a part of microtubule associated proteins (MAPs). They are densely found in neurons and in trace amounts in non-neuronal cells. In brain six isoforms of τ (TAU) proteins are present.
The antibody reacts exclusively with the chemically heterogeneous τ in both the phosphorylated and non-phosphorylated form. The antibody does not react with other MAPs or with tubulin. In immunohistochemical staining, it localizes τ along microtubules in axons, somata, dendrites and astrocytes, and on ribosomes. The antibody may be used for staining of τ in Alzheimer neurofibrillary tangles in sections of human brain tissue.
The best known microtubule associated proteins (MAPs) which copurify with microtubules are MAP2 and Tau. These two proteins are heat stable and stimulate formation of the microtubule polymer from purified tubulin subunits. Tau is chemically heterogenous, however, limited protolysis has demonstrated that the different eletrophoretic species are closely related. Tau is immunologically distinct from the other MAPs, namely MAP1, MAP2 and MAP5. Localization studies have demonstrated that Tau is intimately associated with the filamentous structures which compose the neurofibrillary tangles as found in an Alzheimer′s disease brain.

Immunogen

bovine microtubule-associated proteins (MAPs)

Application

Monoclonal Anti-τ (Tau) antibody has been used:
  • in immunohistology
  • in immunoblotting
  • in dot blot
  • in immunohistochemistry

Mouse monoclonal clone TAU-2 anti-Tau antibody maybe used to study microtubule associated proteins (MAP) expression and cytological localization in various tissue and cell lines, under different developmental and environmental circumstances.

Biochem/physiol Actions

Monoclonal Anti- τ (TAU) is phosphatase independent; it will bind Tau proteins in either their phosphorylated or non-phosphorylated forms. It localizes Tau proteins along microtubules in axons, somata, dendrites, astrocytes and on ribosomes (polysomes). The best-known microtubule associated proteins (MAPs) which copurify with microtubules are MAP2 and Tau. These two proteins are heat stable and stimulate formation of the microtubule polymer from purified tubulin subunits. Tau is immunologically distinct from the other MAPs. Tau is intimately associated with the filamentous structures which compose the neurofibrillary tangles as found in an Alzheimer′s disease brain.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Proteasome inhibition drives HDAC6-dependent recruitment of tau to aggresomes.
Guthrie, et al.
Journal of Molecular Neuroscience, 45, 32-41 (2018)
Huishan Guo et al.
The American journal of pathology, 165(2), 523-531 (2004-07-28)
Previously, we have shown that caspase-6 but not caspase-3 is activated by serum deprivation and induces a protracted cell death in primary cultures of human neurons (LeBlanc AC, Liu H, Goodyer C, Bergeron C, Hammond J: Caspase-6 role in apoptosis...
R E Mrak et al.
Human pathology, 26(8), 816-823 (1995-08-01)
The roles of activated glia and of glial cytokines in the pathogenesis of Alzheimer's disease are reviewed. Interleukin-1 (IL-1), a microglia-derived acute phase cytokine, activates astrocytes and induces expression of the astrocyte-derived cytokine, S100 beta, which stimulates neurite growth (and...
Severity of gliosis in Pick?s disease and frontotemporal lobar degeneration: tau-positive glia differentiate these disorders
Schofield E, et al.
Brain, 126(4), 827-840 (2003)
Cortical limb myoclonus in pathologically proven progressive supranuclear palsy.
Sharon Kemp et al.
Movement disorders : official journal of the Movement Disorder Society, 28(13), 1804-1806 (2013-10-15)
Articles
Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions.
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