T5816

Tricine

BioPerformance Certified, suitable for cell culture, ≥99% (titration)

• Synonym(s): 2-[[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid, N-[Tris(hydroxymethyl)methyl]glycine, N-[Tris(hydroxymethyl)methyl]glycine
• Linear Formula: (HOCH₂)₃CNHCH₂CO₂H
• CAS Number: 5704-04-1
• Molecular Weight: 179.17
• UNSPSC Code: 12161700
• NACRES: NA.25
• PubChem Substance ID: 24900318
• EC Number: 227-193-6
• Beilstein/REAXYS Number: 1937804
• MDL number: MFCD00004277

Properties

• Quality Segment: 400
• grade: BioPerformance Certified
• assay: ≥99% (titration)
• form: crystalline powder
• storage condition: dry at room temperature
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin and total aerobic microbial count, tested
• color: white
• useful pH range: 7.4-8.8
• pKa (25 °C): 8.1
• mp: 187 °C
• cation traces: heavy metals (as Pb): ≤5 ppm
• absorption: ≤0.02 at 290 at 20%
• application(s): diagnostic assay manufacturing; general analytical; life science and biopharma; sample preparation
• SMILES string: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• InChI key: SEQKRHFRPICQDD-UHFFFAOYSA-N

Description

General description

Tricine is a zwitterionic biological buffer commonly used in enzyme assays, electrophoresis, and cell culture research. It has a higher negative charge compared to glycine, which enables it to migrate more rapidly. Tricine′s high ionic strength has the ability to cause more ion movement and less protein movement, resulting in the separation of low molecular weight proteins in lower percent acrylamide gels. It has been observed that Tricine is efficient in the separation of proteins ranging from 1 to 100 kDa through electrophoresis. According to a study, the 25 mmol/L Tricine buffer proved to be the most effective among ten other buffers tested for ATP assays using firefly luciferase. Further, Tricine is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography.

Application

Buffer component for separation of low molecular weight peptides.

Tricine serves as a versatile buffering agent suitable for separating low molecular weight proteins in lower percent acrylamide gels, has demonstrated efficacy in ATP assays employing firefly luciferase, and has shown potential in scavenging hydroxyl radicals during the investigation of radiation-induced membrane damage. Additionally, it has played a role in isolating and purifying ribosomes engaged in the translation of specific mRNA and containing specific peptidyl-tRNA molecules[1]. Tricine has also been employed in the synthesis of RNA oligonucleotides and the fabrication of high-density DNA microarrays, enabling the exploration of mechanisms underlying nucleic acid·ligand interactions and library preparation through photolithography[2].

Features and Benefits

• Suitable as a Buffer component, for Electrophoresis and Protein separation
• Effective Buffering from pH 7.4-8.8 (25 °C) with a pKa of 8.1 (25 °C)
• Tested for Endotoxins and Total Aerobic Microbial Count
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Other Notes

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Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)