T5816

Sigma-Aldrich

Tricine

BioPerformance Certified, suitable for cell culture, ≥99% (titration)

Synonym(s):
N-[Tris(hydroxymethyl)methyl]glycine
Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
Molecular Weight:
179.17
Beilstein/REAXYS Number:
1937804
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

assay

≥99% (titration)

application(s)

cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

useful pH range

7.4 - 8.8

pKa (25 °C)

8.1

mp

187 °C

cation traces

heavy metals (as Pb): ≤5 ppm

absorption

≤0.02 at 290 at 20%

Featured Industry

Diagnostic Assay Manufacturing

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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Related Categories

Packaging

1 kg in poly bottle
100, 500 g in poly bottle

Application

Buffer component for separation of low molecular weight peptides.

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Benjamin Bardiaux et al.
Structure (London, England : 1993), 27(7), 1082-1093 (2019-05-06)
Bacterial type 4a pili are dynamic surface filaments that promote bacterial adherence, motility, and macromolecular transport. Their genes are highly conserved among enterobacteria and their expression in enterohemorrhagic Escherichia coli (EHEC) promotes adhesion to intestinal epithelia and pro-inflammatory signaling. To...
Christian Nilsson et al.
Electrophoresis, 31(3), 459-464 (2010-02-02)
Totally porous lipid-based liquid crystalline nanoparticles were used as pseudostationary phase for capillary electroseparation with LIF detection of proteins at physiological conditions using unmodified cyclic olefin copolymer capillaries (Topas, 6.7 cm effective length). In the absence of nanoparticles, i.e. in...
Thierry Rabilloud
Journal of proteomics, 73(8), 1562-1572 (2010-04-17)
Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations....
Bin Deng et al.
PloS one, 9(7), e102362-e102362 (2014-07-19)
Maresins are a new family of anti-inflammatory and pro-resolving lipid mediators biosynthesized from docosahexaenoic acid (DHA) by macrophages. Here we identified a novel pro-resolving product, 13R,14S-dihydroxy-docosahexaenoic acid (13R,14S-diHDHA), produced by human macrophages. PCR mapping of 12-lipoxygenase (12-LOX) mRNA sequence in...
Hermann Schägger
Nature protocols, 1(1), 16-22 (2007-04-05)
Tricine-SDS-PAGE is commonly used to separate proteins in the mass range 1-100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in...

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