T5941

Trizma^® hydrochloride

BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

• Synonym(s): TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
• Linear Formula: NH₂C(CH₂OH)₃ · HCl
• CAS Number: 1185-53-1
• Molecular Weight: 157.60
• Beilstein: 3675235
• EC Number: 214-684-5
• MDL number: MFCD00012590
• PubChem Substance ID: 24900332
• NACRES: NA.25

Properties

• grade: BioPerformance Certified
• Quality Level: 400
• assay: ≥99.0% (titration)
• form: crystalline powder
• technique(s): cell culture | mammalian: suitable; electrophoresis: suitable
• impurities: DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected; endotoxin and total aerobic microbial count, tested; ≤0.5% water (Karl Fischer)
• useful pH range: 7.0-9.0
• pKa (25 °C): 8.1
• mp: 150-152 °C
• solubility: H₂O: 667 mg/mL
• absorption: ≤0.05 at 290 at 40%
• suitability: suitable for electrophoresis
• application(s): diagnostic assay manufacturing
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Packaging

100, 500, 500, 500 g

1, 1, 5, 50 kg

T5941-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves