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T5941

Sigma-Aldrich

Trizma® hydrochloride

BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

Synonym(s):
TRIS HCl, Tris(hydroxymethyl)aminomethane hydrochloride, TRIS hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein:
3675235
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

assay

≥99.0% (titration)

form

crystalline powder

application(s)

cell culture | mammalian: suitable
electrophoresis: suitable

impurities

DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected
endotoxin and total aerobic microbial count, tested
≤0.5% water (Karl Fischer)

useful pH range

7.0 - 9.0

pKa (25 °C)

8.1

mp

150-152 °C

solubility

H2O: 667 mg/mL

absorption

≤0.05 at 290 at 40%

suitability

suitable for electrophoresis

Featured Industry

Diagnostic Assay Manufacturing

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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Related Categories

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Packaging

1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum
T5941-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Preparation of extracts from prokaryotes.
M Cull et al.
Methods in enzymology, 182, 147-153 (1990-01-01)
Steve D Knutson et al.
Current protocols in chemical biology, 12(2), e82-e82 (2020-05-30)
Adenosine to-inosine (A-to-I) RNA editing is a conserved post-transcriptional modification that is critical for a variety of cellular processes. A-to-I editing is widespread in nearly all types of RNA, directly imparting significant global changes in cellular function and behavior. Dysfunctional...
Nadine Schrode et al.
Nature genetics, 51(10), 1475-1485 (2019-09-25)
The mechanisms by which common risk variants of small effect interact to contribute to complex genetic disorders are unclear. Here, we apply a genetic approach, using isogenic human induced pluripotent stem cells, to evaluate the effects of schizophrenia (SZ)-associated common...
Alexandar Iliev et al.
Acta histochemica, 121(1), 16-28 (2018-10-20)
The hypertrophy of the cardiac muscle is one of the most significant maladaptive mechanisms activated in response to increased workload. It is associated with histological and ultrastructural alterations, changes in the quantitative parameters and the expression of different enzymes. While...
Arun Kumar Tharkeshwar et al.
STAR protocols, 1(3), 100122-100122 (2020-12-31)
Lysosomes are dynamic organelles that serve as regulatory hubs in cellular homeostasis. Changes in lysosome morphology, composition, and turnover are typically linked to disease. These characteristics make enrichment protocols based on biophysical parameters challenging. However, organelle enrichment methods are essential...

Protocols

Oligodt Columns

Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography

10x Blocking Buffer Protocol

Membrane-based blotting applications that employ enzyme conjugates to generate colorimetric or chemiluminescent signal require the use of an added blocking step to decrease the signal generated by non-specific binding.

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

RNAi In Situ

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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