Trizma® hydrochloride

BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

TRIS HCl, Tris(hydroxymethyl)aminomethane hydrochloride, TRIS hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:


BioPerformance Certified

Quality Level


≥99.0% (titration)


crystalline powder


cell culture | mammalian: suitable
electrophoresis: suitable


DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected
endotoxin and total aerobic microbial count, tested
≤0.5% water (Karl Fischer)

useful pH range

7.0 - 9.0

pKa (25 °C)



150-152 °C


H2O: 667 mg/mL


≤0.05 at 290 at 40%


suitable for electrophoresis

Featured Industry

Diagnostic Assay Manufacturing

SMILES string




InChI key


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The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.


1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Preparation of extracts from prokaryotes.
M Cull et al.
Methods in enzymology, 182, 147-153 (1990-01-01)
Nadine Schrode et al.
Nature genetics, 51(10), 1475-1485 (2019-09-25)
The mechanisms by which common risk variants of small effect interact to contribute to complex genetic disorders are unclear. Here, we apply a genetic approach, using isogenic human induced pluripotent stem cells, to evaluate the effects of schizophrenia (SZ)-associated common...
Steve D Knutson et al.
Current protocols in chemical biology, 12(2), e82-e82 (2020-05-30)
Adenosine to-inosine (A-to-I) RNA editing is a conserved post-transcriptional modification that is critical for a variety of cellular processes. A-to-I editing is widespread in nearly all types of RNA, directly imparting significant global changes in cellular function and behavior. Dysfunctional...
Le P Ngo et al.
Nucleic acids research, 48(3), e13-e13 (2019-12-12)
Genotoxicity testing is critical for predicting adverse effects of pharmaceutical, industrial, and environmental chemicals. The alkaline comet assay is an established method for detecting DNA strand breaks, however, the assay does not detect potentially carcinogenic bulky adducts that can arise...
Enrichment of histones from patient samples for mass spectrometry-based analysis of post-translational modifications.
Noberini, et al.
Methods, 184, 19-28 (2020)
Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography
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Membrane-based blotting applications that employ enzyme conjugates to generate colorimetric or chemiluminescent signal require the use of an added blocking step to decrease the signal generated by non-specific binding.
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Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
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<i>In Situ</i> Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition
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