T6025

Tris Acetate-EDTA buffer

BioReagent, suitable for electrophoresis

• Synonym(s): TAE buffer
• NACRES: NA.25
• PubChem Substance ID: 329826837
• UNSPSC Code: 41105319
• MDL number: MFCD00236357

Properties

• InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
• SMILES string: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI key: HGEVZDLYZYVYHD-UHFFFAOYSA-N
• sterility: 0.2 μm filtered
• product line: BioReagent
• form: working solution
• technique(s): electrophoresis: suitable
• impurities: DNase, RNase, Protease, none detected
• suitability: suitable for electrophoresis, suitable for gel electrophoresis (after dilution to working concentration)
• application(s): diagnostic assay manufacturing
• Quality Level: 200

Description

Application

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Tris Acetate-EDTA buffer has been used as run buffer for the agarose gel electrophoresis of human papillomavirus DNA[1] and transcribed RNA samples.[2]

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Solution prepared with 18 megohm water

Other Notes

40 mM Tris acetate, pH approx. 8.3, containing 1 mM EDTA.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)