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Monoclonal Anti-α-Tubulin antibody produced in mouse

enhanced validation

clone B-5-1-2, purified immunoglobulin, buffered aqueous solution

MDL number:

Quality Level

biological source


antibody form

purified immunoglobulin

antibody product type

primary antibodies


B-5-1-2, monoclonal


buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

chicken, kangaroo rat, sea urchin, rat, Chlamydomonas, bovine, human, African green monkey, mouse

enhanced validation

independent ( Antibodies)
Learn more about Antibody Enhanced Validation


~2 mg/mL


immunocytochemistry: 0.5-1 μg/mL using cultured chicken fibroblasts (CFB)
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using total cell extract of human foreskin fibroblast cell line (FS11)





Featured Industry

Research Pathology

UniProt accession no.

shipped in

dry ice

storage temp.


Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

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General description

α -tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α -subunit of tubulin has molecular weight of 50,000.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the hybridoma B-5-1-2 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin).
Tubulin is a heterodimer that consists of α-tubulin and β-tubulin. Both subunits have a molecular weight of approx. 50 kDa and share considerable homology. In addition to α- and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven. Most of these tubulins have distinct subcellular localization and an emerging diverse set of functions. Tubulin is the major building block of microtubules. This intracellular, cylindrical, filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.
Microtubular systems contain at least three α-tubulin isoforms. Two isoforms are coded by two αt-ubulin genes, which are both transcribed and code for extremely similar proteins. The third isoform is generated by post-translational modification.


Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.


Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.


Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in immunoblotting.


200 μL in glass insert

Biochem/physiol Actions

Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).
α-Tubulin is a key regulator of cytoskeletal proteins. It mediates cellular developmental stages such as proliferation, migration, signalling and also maintains the shape of the cell. α-Tubulin controls trafficking, signaling and cellular tensegrity mediated by microtubules. The encoded protein is associated with the development and progression of cancer. α-Tubulin acetylation potentiates the metastatic property of breast cancer. Mutation in TUBA4A is associated with the development of various types of cancers, such as oral cancer, breast cancer, rectal cancer, lung cancer and prostate cancer. In addition, variation in the TUBA4A leads to sporadic amyotrophic lateral sclerosis (ALS).
Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Xiuying Yang et al.
Science signaling, 12(594) (2019-08-15)
The ability of skeletal muscle to switch between lipid and glucose oxidation for ATP production during metabolic stress is pivotal for maintaining systemic energy homeostasis, and dysregulation of this metabolic flexibility is a dominant cause of several metabolic disorders. However...
Arneet L Saltzman et al.
Genes & development, 25(4), 373-384 (2011-02-18)
Alternative splicing (AS) plays a major role in the generation of proteomic diversity and in gene regulation. However, the role of the basal splicing machinery in regulating AS remains poorly understood. Here we show that the core snRNP (small nuclear...
LeDizet M and Piperno G J
Cell Biol., 104, 13-22 (1986)
Dutcher SK, et al.
Current Opinion in Cell Biology, 13, 49-54 (2001)
Specific inhibition of NF-Y subunits triggers different cell proliferation defects.
Benatti P
Nucleic Acids Research, 39(13), 5356-5368 (2011)

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