T6146

Trypan Blue

powder, BioReagent, suitable for cell culture

• Synonym(s): Direct blue 14, Niagara blue reagent, blue live/dead cell assay
• Linear Formula: C₃₄H₂₄N₆O₁₄S₄Na₄
• CAS Number: 72-57-1
• Molecular Weight: 960.81
• EC Number: 200-786-7
• UNSPSC Code: 12352207
• NACRES: NA.75
• PubChem Substance ID: 57654686
• MDL number: MFCD00003969
• Beilstein/REAXYS Number: 4360496

Properties

• SMILES string: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
• InChI key: GLNADSQYFUSGOU-UHFFFAOYSA-J
• product line: BioReagent
• form: powder
• composition: Dye content, ~40%
• packaging: glass bottle of 100 g, glass bottle of 25 g, glass bottle of 5 g
• storage condition: dry at room temperature
• technique(s): cell culture | mammalian: suitable, tissue processing: suitable
• mp: >300 °C (lit.)
• solubility: H₂O: 10 mg/mL
• application(s): cell analysis
• Quality Level: 300
• Gene Information: human ... PTPN1(5770), PTPRF(5792)

Description

General description

The trypan blue dye is an azo dye,[1] derived from toluidine.[2] It is soluble in water and has a molecular weight of 961 g/mol.[1]

Application

Trypan blue has been used:

• in cell viability assay[3]
• to stain plant tissue in order to to visualize cell death[4]

Biochem/physiol Actions

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.[5]

Preparation Note

To make a cell viability solution, add the T6146 Trypan Blue powder to a clean and dry glass or plastic container of appropriate capacity. Add a suitable solvent, such as sterile distilled water, to the container with the Trypan Blue powder. Stir the solution vigorously using a stir bar or a glass rod until the powder has completely dissolved. A 0.4% solution is a common concentration for typical cell viability assessment. For example, to make 100 mL of a 0.4% solution, add 400 milligrams (0.4 g) to 99.6 mL solvent.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H350
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• ppe: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges