T6146

Sigma-Aldrich

Trypan Blue

powder, BioReagent, suitable for cell culture

Synonym(s):
Direct blue 14
Linear Formula:
C34H24N6O14S4Na4
CAS Number:
Molecular Weight:
960.81
Colour Index Number:
23850
Beilstein/REAXYS Number:
4360496
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

Quality Level

product line

BioReagent

form

powder

composition

Dye content, ~40%

packaging

glass bottle of 100 g
glass bottle of 25 g
glass bottle of 5 g

storage condition

dry at room temperature

application(s)

cell analysis: suitable
cell culture | mammalian: suitable
tissue processing: suitable

mp

>300 °C (lit.)

solubility

H2O: 10 mg/mL

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

Gene Information

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General description

The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.

Application

Trypan blue has been used:
  • in cell viability assay
  • to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
  • to stain plant tissue in order to to visualize cell death

Packaging

5, 25, 100 g in glass bottle

Biochem/physiol Actions

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

hazcat

Carc. 1B

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 3

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

A histological analysis of lens capsules stained with trypan blue for capsulorrhexis in phacoemulsification cataract surgery
Singh AJ, et al.
Eye (London, England), 17(5), 567-567 (2003)
Plant tissue trypan blue staining during phytopathogen infection.
Fernandez NB, et al.
Bio-protocol (2016)
Acanthamoeba: Biology and Pathogenesis, 85-85 (2009)
Trypan blue exclusion test of cell viability
Strober W
Current Protocols in Immunology, 21(1), A-3B (1997)
Knockdown of PSMB7 induces autophagy in cardiomyocyte cultures: possible role in endoplasmic reticulum stress
Kyrychenko VO. et al.
Pathobiology, 81(1), 8-14 (2014)
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