T6277

Sigma-Aldrich

Monoclonal Anti-Troponin T antibody produced in mouse

clone JLT-12, ascites fluid

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

JLT-12, monoclonal

contains

15 mM sodium azide

species reactivity

rat, chicken, rabbit, bovine

application(s)

immunohistochemistry: suitable
western blot: 1:200 using rabbit total skeletal muscle extract

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

rat ... Tnnt1(171409), Tnnt2(24837), Tnnt3(24838)

General description

Troponin T is a vital subunit of troponin. Troponin T is a comma or rod shaped protein with a length of 185-205 Å. Troponin T is positioned in the actin helix groove and is extended along the filament of troponin. Life span of serum troponin T is 120 minutes. N- terminal end of the protein is enriched in negatively charged residues and C- terminal end is enriched with positively charged residues. This uneven distribution of charges in troponin T facilitates its aggregation at physiological salt concentration. In humans, cardiac troponin T exists in four isoforms, out of which three are expressed in the fetus and one isoform is expressed in the adult heart.
Monoclonal Anti-Troponin T (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

Troponin T from rabbit skeletal muscle.

Application

Monoclonal Anti-Troponin T antibody produced in mouse has been used in following studies:
  • Western blot analysis.
  • Immunocytochemical analysis.
  • Immunofluorescence.
Monoclonal Anti-Troponin T antibody produced in mouse has been used in immunostaining.

Biochem/physiol Actions

Troponin T is a subunit of troponin, which facilitates binding of troponin to tropomyosin. It also plays a crucial role in muscle contraction. Cardiac troponin T acts as a potential marker for the detection of acute myocardial infarction (MI). Apart from facilitating binding of troponin components to the actin-tropomyosin filament, it is has an essential role in the regulation of actomyosin ATPase activity. Cardiac troponin T gene mutation leads to hypertrophic cardiomyopathy.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
J C Moolman et al.
Journal of the American College of Cardiology, 29(3), 549-555 (1997-03-01)
This study was designed to verify initial observations of the clinical and prognostic features of hypertrophic cardiomyopathy caused by cardiac tropnin T gene mutations. The most common cause of sudden cardiac death in the young is hypertrophic cardiomyopathy, which is...
Dynamics of the skeletal muscle secretome during myoblast differentiation
Henningsen J, et al.
Molecular and Cellular Proteomics, 9(11), 2482-2496 (2010)
C Lindon et al.
The Journal of cell biology, 140(1), 111-118 (1998-02-14)
Myf5 is the earliest-known muscle-specific factor to be expressed in vivo and its expression is associated with determination of the myoblast lineage. In C2 cells, we show by immunocytolocalization that Myf5 disappears rapidly from cells in which the differentiation program...
V L Filatov et al.
Biochemistry. Biokhimiia, 64(9), 969-985 (1999-10-16)
This review discusses the structure and properties of the isolated components of troponin, their interaction, and the mechanisms of regulation of contractile activity of skeletal and cardiac muscle. Data on the structure of troponin C in crystals and in solution...
Lori A Walker et al.
American journal of physiology. Heart and circulatory physiology, 301(3), H832-H840 (2011-05-31)
Right ventricular (RV) failure is one of the strongest predictors of mortality both in the presence of left ventricular decompensation and in the context of pulmonary vascular disease. Despite this, there is a limited understanding of the biochemical and mechanical...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.