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T6400

Sigma-Aldrich

Tris-Borate-EDTA buffer

5× Concentrate

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Synonym(s):
TBE buffer
MDL number:
PubChem Substance ID:

Quality Level

sterility

0.2 μm filtered

form

solution

impurities

DNase and RNase, none detected

pH

8.2-8.4 (25 °C)

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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This Item
T4415T9650T3913
Tris-Borate-EDTA buffer 5× Concentrate

Sigma-Aldrich

T6400

Tris-Borate-EDTA buffer

-
Tris-Borate-EDTA buffer BioReagent, suitable for electrophoresis, 10× concentrate

Sigma-Aldrich

T4415

Tris-Borate-EDTA buffer

Essential+ Grade
Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

Sigma-Aldrich

T9650

Tris Acetate-EDTA buffer

Essential+ Grade
Tris-Borate-EDTA buffer 5× concentrate, powder blend

Sigma-Aldrich

T3913

Tris-Borate-EDTA buffer

-
form

solution

form

solution

form

solution

form

powder blend

impurities

DNase and RNase, none detected

impurities

DNase and RNase, none detected

impurities

-

impurities

DNase, RNase and NICKase, none detected

pH

8.2-8.4 (25 °C)

pH

-

pH

-

pH

8.1-8.5 (25 °C, 5 ×)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Ready for use in gel electrophoresis after dilution to working concentrations.
Tris-Borate-EDTA buffer has been used in the electrophoresis of the plasmid extracted from activated sludge.
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

Supplied in dispenser with a spigot.

Other Notes

0.445 M Tris borate, pH approx. 8.3, containing 0.01 M EDTA.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
Solution prepared with 18 megohm water

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