All Photos(7)

T6557

Sigma-Aldrich

Monoclonal Anti-γ-Tubulin antibody produced in mouse

clone GTU-88, ascites fluid

Synonym(s):
Anti-γ-Tubulin antibody, Mouse monoclonal
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GTU-88, monoclonal

mol wt

antigen 48 kDa

contains

15 mM sodium azide

species reactivity

bovine, mouse, rat, chicken, Xenopus, human, hamster, canine

technique(s)

immunocytochemistry: 1:5,000-1:10,000 using HeLa cells
indirect ELISA: suitable
western blot: 1:10,000 using cultured chicken fibroblast extract

isotype

IgG1

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)

Looking for similar products? Visit Product Comparison Guide

General description

γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. γ-Tubulin is mapped to human chromosome 17q21.2 and codes for a member of the tubulin family. Human TUBG1 transcript is widely expressed in preimplantation embryos and brain.
Monoclonal Anti-γ-Tubulin (mouse IgG1 isotype) is derived from the GTU-88 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.
Monoclonal Anti-γ-Tubulin recognizes an epitope located in the N-terminal amino acids of γ-tubulin (48 kDa). Cross reactivity has been observed with human, bovine, dog, hamster, rat, mouse, chicken, and Xenopus γ-tubulin.

Immunogen

synthetic γ-tubulin peptide, conjugated to KLH

Application

Monoclonal Anti-γ-Tubulin antibody has been used in western blotting, indirect immunofluorescence (IF) and immunofluorescence staining.
Monoclonal Anti-γ-Tubulin is also suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells and in ELISA.
Monoclonal Anti-gamma-Tubulin is suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells, and in ELISA.

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. It functions as the microtubule nucleator at the MTOC. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. Overexpression of γ-tubulin is observed in lung cancer. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Physical form

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Association of Aurora A and gamma-tubulin expression in astrocytomas and patient survival.
Tsai HP, et al.
Neurological Research, 36(8), 746-751 (2014)
γ-Tubulin is a centrosomal protein required for cell cycle-dependent microtubule nucleation.
Joshi HC, et al.
Nature, 356(6364), 80-80 (1992)
Human NEIL1 localizes with the centrosomes and condensed chromosomes during mitosis.
Hildrestrand GA, et al.
DNA Repair, 6(10), 1425-1433 (2007)
Interaction of amyotrophic lateral sclerosis (ALS)-related mutant copper-zinc superoxide dismutase with the dynein-dynactin complex contributes to inclusion formation.
Strom AL, et al.
The Journal of Biological Chemistry, 283(33), 22795-22805 (2008)
Overexpression of γ-tubulin in non-small cell lung cancer.
Maounis NF, et al.
Histology and Histopathology, 27(7), 1183-1183 (2012)

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service