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T6557

Sigma-Aldrich

Anti-γ-Tubulin antibody, Mouse monoclonal

clone GTU-88, ascites fluid

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Synonym(s):
Anti-γ-Tubulin antibody, Mouse monoclonal
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GTU-88, monoclonal

mol wt

antigen 48 kDa

contains

15 mM sodium azide

species reactivity

rat, hamster, chicken, human, bovine, canine, Xenopus, mouse

technique(s)

immunocytochemistry: 1:5,000-1:10,000 using HeLa cells
indirect ELISA: suitable
western blot: 1:10,000 using cultured chicken fibroblast extract

isotype

IgG1

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)

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This Item
T5326T3320T5168
Gene Information

human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)

Gene Information

human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)

Gene Information

human ... TUBG1(7283), TUBG2(27175)
rat ... Tubg1(252921), Tubg2(680991)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

clone

GTU-88, monoclonal

clone

GTU-88, monoclonal

clone

polyclonal

clone

B-5-1-2, monoclonal

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

antibody form

ascites fluid

antibody form

purified antibody (purified from hybridoma cell culture)

antibody form

IgG fraction of antiserum

antibody form

ascites fluid

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General description

γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. γ-Tubulin is mapped to human chromosome 17q21.2 and codes for a member of the tubulin family. Human TUBG1 transcript is widely expressed in preimplantation embryos and brain.
Monoclonal Anti-γ-Tubulin (mouse IgG1 isotype) is derived from the GTU-88 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

Monoclonal Anti-γ-Tubulin recognizes an epitope located in the N-terminal amino acids of γ-tubulin (48 kDa). Cross reactivity has been observed with human, bovine, dog, hamster, rat, mouse, chicken, and Xenopus γ-tubulin.
The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.

Immunogen

synthetic γ-tubulin peptide, conjugated to KLH

Application

Monoclonal Anti-γ-Tubulin antibody has been used in western blotting, indirect immunofluorescence (IF) and immunofluorescence staining.
Monoclonal Anti-γ-Tubulin is also suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells and in ELISA.
Monoclonal Anti-gamma-Tubulin is suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells, and in ELISA.

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. It functions as the microtubule nucleator at the MTOC. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. Overexpression of γ-tubulin is observed in lung cancer. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Physical form

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Interaction of amyotrophic lateral sclerosis (ALS)-related mutant copper-zinc superoxide dismutase with the dynein-dynactin complex contributes to inclusion formation.
Strom AL, et al.
The Journal of Biological Chemistry, 283(33), 22795-22805 (2008)
Overexpression of γ-tubulin in non-small cell lung cancer.
Maounis NF, et al.
Histology and Histopathology, 27(7), 1183-1183 (2012)
Human mineralocorticoid receptor (MR) gene haplotypes modulate MR expression and transactivation: implication for the stress response.
Van Leeuwen N, et al.
Psychoneuroendocrinology, 36(5), 699-709 (2011)
γ-Tubulin is a centrosomal protein required for cell cycle-dependent microtubule nucleation.
Joshi HC, et al.
Nature, 356(6364), 80-80 (1992)
Association of Aurora A and gamma-tubulin expression in astrocytomas and patient survival.
Tsai HP, et al.
Neurological Research, 36(8), 746-751 (2014)

Articles

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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