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Thrombin from bovine plasma

lyophilized powder, 600-2,000 NIH units/mg protein (biuret)

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Factor IIa
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:


lyophilized powder

Quality Level

specific activity

600-2,000 NIH units/mg protein (biuret)

mol wt

heavy chain ~33 kDa
light chain ~5 kDa

UniProt accession no.


diagnostic assay manufacturing

storage temp.


Gene Information

cow ... F2(280685)

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General description

Thrombin is a sodium-activated type II enzyme. It contains two anion binding exosites, ABE-I and ABE-II. The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Bovine a-thrombin consists of a light chain (A chain) and a heavy chain (B chain). These two chains are joined by one disulfide bond. The B chain of a-thrombin includes a carbohydrate portion.


Thrombin from bovine plasma has been used to study its effect on the perinatal rat subventricular zone cells and oligodendrocyte precursor cell proliferation, differentiation, and migration in culture. It has also been used in fibrin degradation assay to measure nattokinase activity.
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess global haemostasis and point of care testing.

Biochem/physiol Actions

Thrombin is a proteolytic enzyme critical in the blood clotting process and activates clotting factors V, VIII, XI, and XII. Thrombin promotes platelet aggregation. Therefore, thrombin is the final coagulation protease in hemostasis, promoting both procoagulant and anticoagulant effects. It is used to treat bleeding from capillaries and small venules.
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Unit Definition

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

View more information on thrombin at


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Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3


Respiratory system

Storage Class

11 - Combustible Solids




Not applicable


Not applicable

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Thrombin as a Target
xPharm: The Comprehensive Pharmacology Reference null
Thrombin as an Agent
xPharm: The Comprehensive Pharmacology Reference null
Congenital and Acquired Hypercoagulable Syndromes
The Vein Book, 339-346 (2007)
Y Dargaud et al.
Haemophilia : the official journal of the World Federation of Hemophilia, 18 Suppl 4, 81-88 (2012-06-29)
The evaluation of the coagulation profile has used so far either clotting-based or chromogenic assays with different endpoints. Clotting-based techniques are the most used worldwide, and they certainly are useful for diagnosis of clotting factor deficiencies. However, the information provided
Akiomi Takano et al.
Investigative ophthalmology & visual science, 47(5), 2075-2079 (2006-04-28)
To investigate the effects of intravitreal injection of nattokinase (subtilisin NAT), a serine protease that is produced by Bacillus subtilis (natto), for induction of posterior vitreous detachment (PVD). Different doses of nattokinase (1, 0.1, or 0.01 fibrin-degradation units [FU]) or


Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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