T6824
pPolh-FLAG-1 Transfer Vector
Synonym(s):
ddddk tag, dykddddk tag
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 20 μg | Check Cart for Availability | $644.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
41106621
$644.00
Check Cart for Availability
General description
The pPolh-FLAG-1 vector contains the strong viral polyhedrin (polh) promoter for high-level expression of target genes during the very late phase of infection. The vector also contains a high copy bacterial origin of replication and an ampicillin resistance gene (ampr) for easy propagation in Escherichia coli host cells. Following co-transfection with linear baculovirus DNA into insect cells, allelic replacement between homologous viral sequences (AcNPV ORF 603 and ORF 1629) in the vector and the baculovirus DNA transfers the FLAG-target gene fusion sequence into the viral genome.
Application
pPolh-FLAG-1 is a 5575 bp baculovirus transfer vector used for producing FLAGÒ fusion proteins in insect cells. The vector is designed to be compatible with most baculoviral DNA systems that require the essential gene ORF1629 for complementation of lethal deletions and the recovery of viable recombinant virus. It is also a transfer vector for generation of recombinant Baculovirus and expression of N-terminal FLAG® fusions in insect cells.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
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| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
baculovirus recombinant vector, N-terminal FLAG fusions, insect recombinant protein expression | - | baculovirus recombinant vector, N-terminal FLAG fusions, insect recombinant protein expression | - |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Baculovirus Expression Vectors
O'Reilly, D. R., et al.
Baculovirus Expression Vectors: A Laboratory Manual (1994)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T6824-20UG | 04061838072894 |