MilliporeSigma
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T6824

Sigma-Aldrich

pPolh-FLAG™-1 Transfer Vector

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Synonym(s):
ddddk tag, dykddddk tag
NACRES:
NA.32

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

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shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

baculovirus recombinant vector, N-terminal FLAG fusions, insect recombinant protein expression

baculovirus recombinant vector, N-terminal FLAG fusions, insect recombinant protein expression

baculovirus recombinant vector, N-terminal FLAG fusions, insect recombinant protein expression

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The pPolh-FLAG-1 vector contains the strong viral polyhedrin (polh) promoter for high-level expression of target genes during the very late phase of infection. The vector also contains a high copy bacterial origin of replication and an ampicillin resistance gene (ampr) for easy propagation in Escherichia coli host cells. Following co-transfection with linear baculovirus DNA into insect cells, allelic replacement between homologous viral sequences (AcNPV ORF 603 and ORF 1629) in the vector and the baculovirus DNA transfers the FLAG-target gene fusion sequence into the viral genome.

Application

pPolh-FLAG-1 is a 5575 bp baculovirus transfer vector used for producing FLAGÒ fusion proteins in insect cells. The vector is designed to be compatible with most baculoviral DNA systems that require the essential gene ORF1629 for complementation of lethal deletions and the recovery of viable recombinant virus. It is also a transfer vector for generation of recombinant Baculovirus and expression of N-terminal FLAG® fusions in insect cells.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC
pPolh-FLAG is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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