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T7193

Sigma-Aldrich

Trizma® Pre-set crystals

BioPerformance Certified, pH 7.0, average Mw 154.8

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Synonym(s):
Tris crystals
NACRES:
NA.25

grade

BioPerformance Certified

Agency

suitable for EPA 537.1

form

crystalline powder

mol wt

average Mw 154.8

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, NICKase, none detected
endotoxin, tested
total aerobic microbial count, tested
≤0.05 A290
≤0.5% water (Karl Fischer)
≤5 ppm Heavy metals (as Pb)

pH

7.00 (25 °C, 0.1 M)(lit.)
7.0

useful pH range

7.0-9.0

solubility

water: 0.667 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

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Trizma® Pre-set crystals BioPerformance Certified, pH 7.0, average Mw 154.8

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form

crystalline powder

form

crystalline powder

form

crystalline powder

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, NICKase, none detected, endotoxin, tested, total aerobic microbial count, tested, ≤0.05 A290, ≤0.5% water (Karl Fischer), ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, Protease, NICKase, none detected, endotoxin, tested, total aerobic microbial count, tested, ≤0.05 A290, ≤0.5% water (Karl Fischer), ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, Protease, NICKase, none detected, endotoxin, tested, total aerobic microbial count, tested, ≤0.05 A290, ≤0.5% water (Karl Fischer), ≤5 ppm Heavy metals (as Pb)

impurities

DNase, RNase, Protease, NICKase, none detected, endotoxin, tested, total aerobic microbial count, tested, ≤0.05 A290, ≤0.5% water (Karl Fischer), ≤5 ppm Heavy metals (as Pb)

pH

7.00 (25 °C, 0.1 M)(lit.)

pH

7.20 (25 °C, 0.1 M)(lit.)

pH

7.70 (25 °C, 0.1 M)(lit.)

pH

7.60 (25 °C, 0.1 M)(lit.)

solubility

water: 0.667 g/mL, clear, colorless

solubility

water: 0.66 g/mL, clear, colorless

solubility

water: 0.66 g/mL, clear, colorless

solubility

water: 0.66 g/mL, clear, colorless

Application

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Other Notes

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.
Certain pH electrodes do not give accurate results with Tris buffers.

Preparation Note

Trizma solutions can be autoclaved.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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