All Photos(7)

T7451

Sigma-Aldrich

Monoclonal Anti-Acetylated Tubulin antibody produced in mouse

clone 6-11B-1, purified from hybridoma cell culture

Synonym(s):
Acetylated Tubulin Antibody - Monoclonal Anti-Acetylated Tubulin antibody produced in mouse, Acetylated Tubulin Antibody
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6-11B-1, monoclonal

form

buffered aqueous solution

species reactivity

plant, hamster, rat, mouse, human, pig, monkey, frog, invertebrates, bovine, protista, chicken

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

application(s)

dot blot: suitable
electron microscopy: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: 0.03-0.06 μg/mL using total rat brain extract

isotype

IgG2b

conjugate

unconjugated

Featured Industry

Research Pathology

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Related Categories

General description

Monoclonal Anti-Acetylated Tubulin (mouse IgG2b isotype) is derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin). Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 50,000 and share considerable homology.
α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α -tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.

Specificity

The antibody has been used to detect acetylated α-tubulins from many organisms including protista, plants, invertebrates, and vertebrates. The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corre­sponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila, and yeast. Occasionally, the epitope recognized by the antibody may be absent or masked, as it is in the rat kangaroo epithelial-like cell line PtK2.

Immunogen

acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).

Application

Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has been used in western blot and immunohistochemistry.
Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has also been used in quantitative dot blot, immunofluorescence, Western blot, immunocytochemistry, enzyme linked immunosorbent assay (ELISA), solid phase radioimmunoassay (RIA) and electron microscopy.

Biochem/physiol Actions

Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

All tubulins are not alike: heterodimer dissociation differs among different biological sources
Montecinos-Franjola F, et al.
The Journal of biological chemistry, 9(12), jbc-RA119 (2019)
3D spheroid model of mIMCD3 cells for studying ciliopathies and renal epithelial disorders
Giles RH, et al.
Nature Protocols, 9(12), 2725-2725 (2014)
M LeDizet et al.
Proceedings of the National Academy of Sciences of the United States of America, 84(16), 5720-5724 (1987-08-01)
An acetylation site of Chlamydomonas axonemal alpha-tubulins was identified near, or within, the binding site of 6-11B-1, a monoclonal antibody specific for posttranslationally acetylated alpha-tubulins. In a first approach, axonemal proteins were hydrolyzed by formic acid, cyanogen bromide, or chymotrypsin...
G Piperno et al.
The Journal of cell biology, 104(2), 289-302 (1987-02-01)
The subcellular distribution of microtubules containing acetylated alpha-tubulin in mammalian cells in culture was analyzed with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. Cultures of 3T3, HeLa, and PtK2 cells were grown on coverslips and observed by immunofluorescence microscopy...
Heterozygous loss-of-function SEC61A1 mutations cause autosomal-dominant tubulo-interstitial and glomerulocystic kidney disease with anemia
Bolar NA, et al.
American Journal of Human Genetics, 99(1), 174-187 (2016)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service