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T7513

Sigma-Aldrich

Thrombin from bovine plasma

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

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Synonym(s):
Factor IIa
CAS Number:
9002-04-4
Enzyme Commission number:
3.4.21.5 (BRENDA, IUBMB)
EC Number:
232-648-7
MDL number:
MFCD00166053
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

≥2,000 NIH units/mg protein (E1%/280 = 19.5)

mol wt

heavy chain ~33 kDa
light chain ~5 kDa

UniProt accession no.

P00735

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

cow ... F2(280685)

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1 of 4

This Item
T6634T1063T6884
Sigma-Aldrich

Sigma-Aldrich

T7513

Thrombin from bovine plasma

Sigma-Aldrich

Sigma-Aldrich

T6634

Thrombin from bovine plasma

Thrombin from human plasma lyophilized powder, ≥2800 NIH units/mg protein (E1%/280, 18.3)

Sigma-Aldrich

T1063

Thrombin from human plasma

Thrombin from human plasma lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280, 18.3)

Sigma-Aldrich

T6884

Thrombin from human plasma

specific activity

≥2,000 NIH units/mg protein (E1%/280 = 19.5)

specific activity

600-2,000 NIH units/mg protein (biuret)

specific activity

≥2800 NIH units/mg protein (E1%/280, 18.3)

specific activity

≥2,000 NIH units/mg protein (E1%/280, 18.3)

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

mol wt

37.4 kDa

mol wt

37.4 kDa

UniProt accession no.

P00735

UniProt accession no.

P00735

UniProt accession no.

P00734

UniProt accession no.

P00734

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Unit Definition

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

inhibitor

Product No.
Description
Pricing

substrate

Product No.
Description
Pricing

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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25G
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1000309185

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Articles

Thrombin Factor IIa

Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

Protocols

Thrombin from Bovine Plasma

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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