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T7527

Sigma-Aldrich

Tris-Borate-EDTA buffer

BioReagent, for molecular biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

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Synonym(s):
TBE buffer
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

product line

BioReagent

form

powder blend

impurities

DNase and RNase, none detected

pH

8.1-8.5 (5 ×)

solubility

water: 85.1 g/L, clear, colorless

suitability

suitable for electrophoresis

application(s)

diagnostic assay manufacturing

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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This Item
T3913T8280T4038
Tris-Borate-EDTA buffer BioReagent, for molecular biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Sigma-Aldrich

T7527

Tris-Borate-EDTA buffer

Essential+ Grade
Tris-Borate-EDTA buffer 5× concentrate, powder blend

Sigma-Aldrich

T3913

Tris-Borate-EDTA buffer

-
Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Sigma-Aldrich

T8280

Tris Acetate-EDTA buffer

Essential+ Grade
Tris Acetate-EDTA buffer DNase and RNase, none detected, BioReagent, suitable for electrophoresis, 10× concentrate

Sigma-Aldrich

T4038

Tris Acetate-EDTA buffer

Essential+ Grade
product line

BioReagent

product line

-

product line

BioReagent

product line

BioReagent

form

powder blend

form

powder blend

form

powder blend

form

powder blend

impurities

DNase and RNase, none detected

impurities

DNase, RNase and NICKase, none detected

impurities

DNase and RNase, none detected

impurities

DNase and RNase, none detected

pH

8.1-8.5 (5 ×)

pH

8.1-8.5 (25 °C, 5 ×)

pH

8.1-8.5

pH

8.1-8.5

solubility

water: 85.1 g/L, clear, colorless

solubility

water: 85.1 g/L, clear, colorless

solubility

-

solubility

water: 64.2 g/L, clear, colorless

Application

Ready for use in gel electrophoresis after dilution to working concentrations.
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

1L poly bottle contains a powder blend that can be dissolved and reconstituted within the bottle to prepare one liter of a 5× concentrate.
4L poly bottle contains a powder blend that can be dissolved and reconstituted to prepare four liters of a 5× concentrate.


Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Reconstitution

Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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1000309185

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In Northern Ireland over the last 7 years, there is a mean of 41.9 laboratory reports per annum of human gastrointestinal infection (range 19-54) caused by Escherichia coli O157:H7. In the preceding years 1992-1996, reports were 5.4 per annum, whereas
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Proceedings of the National Academy of Sciences of the United States of America, 106(37), 15549-15554 (2009-09-01)
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Protocols

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

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