T7701
TargeTron™ Vector pJIR750ai
Expression Vector for Bacterial Gene Knockout
Sign Into View Organizational & Contract Pricing
All Photos(1)
Select a Size
Change View
2 μG
$206.00
About This Item
UNSPSC Code:
41106609
NACRES:
NA.51
Recommended Products
storage temp.
−20°C
General description
TargeTron™ vector pJIR750ai is a 10,262 bp expression vector intended for use with the TargeTron Gene Knockout System, Catalog Number TA0100. This circularized vector can be used for targeted gene knockouts in gram-positive bacteria such as Clostridium perfringens type A.1 Expression of the group II intron RNA is under the control of the β-2 toxin gene promoter, cpb2.1 As supplied, the pJIR750ai vector is re-targeted to the α-toxin (plc) gene. α-Toxin, a phospholipase C, is an extracellular toxin produced by all C. perfringens isolates.1 For validation in a specific C. perfringens strain, this vector can be used directly to knockout plc without any further modifications using the enclosed protocol. In order to re-target this vector to knockout other genes, the plc specific IBS-EBS fragment (350 bp) between the Hind III and BsrG I sites can be cut out and replaced with another gene specific fragment. For re-targeting protocols, refer to the TargeTron Gene Knockout System User Guide, Catalog Number TA0100, at sigma-aldrich.com.
Legal Information
TargeTron is a trademark of InGex, LLC
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
