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MilliporeSigma

T7816

Sigma-Aldrich

Monoclonal Anti-β-Tubulin I antibody produced in mouse

clone SAP.4G5, ascites fluid

Synonym(s):

Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5

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100 μL
$552.00
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$727.00

$552.00

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About This Item

MDL number:
MFCD02095890
UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SAP.4G5, monoclonal

mol wt

antigen ~55 kDa

species reactivity

Xenopus, chicken, human, hamster, canine, mouse, bovine, rat

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:20,000 using cultured chicken fibroblasts

isotype

IgG1

UniProt accession no.

P07437

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB(203068)
mouse ... Tubb4(22153)

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This Item
T5293T8535T5076
Monoclonal Anti-β-Tubulin I antibody produced in mouse clone SAP.4G5, ascites fluid

Sigma-Aldrich

T7816

Monoclonal Anti-β-Tubulin I antibody produced in mouse

Monoclonal Anti-β-Tubulin antibody produced in mouse clone 2-28-33, ascites fluid

Sigma-Aldrich

T5293

Monoclonal Anti-β-Tubulin antibody produced in mouse

Monoclonal Anti-β-Tubulin I+II antibody produced in mouse clone JDR.3B8, ascites fluid

Sigma-Aldrich

T8535

Monoclonal Anti-β-Tubulin I+II antibody produced in mouse

Anti-β-Tubulin Isotype III antibody, Mouse monoclonal clone SDL.3D10, purified from hybridoma cell culture

Sigma-Aldrich

T5076

Anti-β-Tubulin Isotype III antibody, Mouse monoclonal

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Gene Information

human ... TUBB(203068)
mouse ... Tubb4(22153)

Gene Information

human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)
rat ... Tubb2(29212), Tubb2c(296554)

Gene Information

human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383)
mouse ... Tubb1(545486), Tubb2a(22151), Tubb2c(227613)
rat ... Tubb2b(291081), Tubb2c(296554)

Gene Information

human ... TUBB3(10381)
mouse ... Tubb3(22152)
rat ... Tubb3(246118)

species reactivity

Xenopus, chicken, human, hamster, canine, mouse, bovine, rat

species reactivity

Ciona intestinalis, rat, chicken, Caenorhabditis elegans, Strongylocentrotus purpuratus, bovine, human

species reactivity

bovine, pig, chicken, human, rat, mouse

species reactivity

mouse, rat, human, boar, bovine

clone

SAP.4G5, monoclonal

clone

2-28-33, monoclonal

clone

JDR.3B8, monoclonal

clone

SDL.3D10, monoclonal

General description

Tubulin a 100kD protein is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-β-tubulin I antibody can be used in ELISA to localize β tubulin isotype I and can be used in immunoblotting to localize cultured human and animal cells. Mouse anti-β-tubulin I antibody reacts specifically with an epitope found in the c-terminal sequence of β tubulin of human, rat, dog, bovine, hamster, mouse, chicken and xenopus

Immunogen

synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype I, conjugated to BSA.

Application

Monoclonal anti-β-tubulin I antibody (diluted 1: 4,000) can be used as a primary antibody in immunoprecipitation and immunoblotting. It can also be used in western blot analysis and retroviral infection.

Biochem/physiol Actions

Recognizes an epitope located in the C-terminal sequence of β-tubulin isotype I. No reactivity with other tubulin isotypes is observed.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000439644
0000439644
0000397445
0000348086
0000348086

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Richard G James et al.
PloS one, 7(12), e50457-e50457 (2012-12-12)
The Wnt/ß-catenin signaling pathway controls important cellular events during development and often contributes to disease when dysregulated. Using high throughput screening we have identified a new small molecule inhibitor of Wnt/ß-catenin signaling, WIKI4. WIKI4 inhibits expression of ß-catenin target genes
M C Roach et al.
Cell motility and the cytoskeleton, 39(4), 273-285 (1998-05-15)
Tubulin, the subunit protein of microtubules, is an alpha/beta heterodimer. In many organisms, both alpha and beta consist of various isotypes. Although the isotypes differ in their tissue distributions, the question of whether the isotypes perform different functions in vivo
Ohad Tarcic et al.
Cell reports, 14(6), 1462-1476 (2016-02-09)
Factors linking inflammation and cancer are of great interest. We now report that the chromatin-targeting E3 ubiquitin ligase RNF20/RNF40, driving histone H2B monoubiquitylation (H2Bub1), modulates inflammation and inflammation-associated cancer in mice and humans. Downregulation of RNF20 and H2Bub1 favors recruitment
Yosef Buganim et al.
Cancer research, 66(22), 10750-10759 (2006-11-17)
Mutations in p53 are ubiquitous in human tumors. Some p53 mutations not only result in loss of wild-type (WT) activity but also grant additional functions, termed "gain of function." In this study, we explore how the status of p53 affects
Richard G James et al.
The Journal of biological chemistry, 288(48), 34658-34670 (2013-10-12)
Advances in phosphoproteomics have made it possible to monitor changes in protein phosphorylation that occur at different steps in signal transduction and have aided the identification of new pathway components. In the present study, we applied this technology to advance
View All Related Papers

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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Questions

  1. Hola: Tenemos este anticuerpo en uso habitual Beta-Tubulina Ref: T7816. Necesito saber la concentración del producto. Gracias

    1 answer

    1. This product is a mouse ascites fluid. The concentration may range from 3.0 to 6.0 mg/mL and is reported in the lot-specific Certificate of Analysis. Please see the link below to review a sample or lot-specific certificate:
      https://www.sigmaaldrich.com/product/sigma/t7816#product-documentation

      Please see the following link to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/125/087/t7816dat.pdf

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