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T7902

Sigma-Aldrich

Thermolysin from Geobacillus stearothermophilus

powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture

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Synonym(s):
Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease
CAS Number:
9073-78-3
Enzyme Commission number:
3.4.24.27 (BRENDA, IUBMB)
EC Number:
232-973-4
MDL number:
MFCD00165549
NACRES:
NA.75

biological source

Geobacillus stearothermophilus

Quality Level

200

product line

BioReagent

form

powder

specific activity

30-350 units/mg protein (E1%/280)

mol wt

34.6 kDa

purified by

crystallization

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

storage temp.

−20°C

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T7902

Thermolysin from Geobacillus stearothermophilus

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P1512

Thermolysin from Geobacillus stearothermophilus

Trypsin from bovine pancreas essentially salt-free, lyophilized powder, ≥9,000 BAEE units/mg protein, BioReagent, suitable for cell culture

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Trypsin from bovine pancreas

Collagenase from Clostridium histolyticum lyophilized powder (from 0.2μm filtered solution), 0.5-5.0 FALGPA units/mg solid, suitable for cell culture

Sigma-Aldrich

C9722

Collagenase from Clostridium histolyticum

form

powder

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder (from 0.2μm filtered solution)

specific activity

30-350 units/mg protein (E1%/280)

specific activity

30-350 units/mg protein (E1%/280)

specific activity

≥9,000 BAEE units/mg protein

specific activity

0.5-5.0 FALGPA units/mg solid

mol wt

34.6 kDa

mol wt

34.6 kDa by amino acid sequence

mol wt

23.8 kDa

mol wt

-

purified by

crystallization

purified by

crystallization

purified by

-

purified by

-

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

Application

A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Physical form

lyophilized powder containing calcium and sodium acetate buffer salts

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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Allen F Ryan et al.
Frontiers in genetics, 11, 358-358 (2020-05-01)
Single-cell transcriptomics was used to profile cells of the normal murine middle ear. Clustering analysis of 6770 transcriptomes identified 17 cell clusters corresponding to distinct cell types: five epithelial, three stromal, three lymphocyte, two monocyte, two endothelial, one pericyte and
Yuichi Kawasaki et al.
Journal of biochemistry, 153(1), 85-92 (2012-10-23)
The thermolysin variant G8C/N60C/S65P in which the triple mutation in the N-terminal domain, Gly8→Cys/Asn60→Cys/Ser65→Pro, is undertaken increases stability [Yasukawa, K. and Inouye, K. (2007) Improving the activity and stability of thermolysin by site-directed mutagenesis. Biochim. Biophys. Acta 1774, 1281-1288] and
Taha A Jan et al.
Cell reports, 36(2), 109358-109358 (2021-07-15)
The utricle is a vestibular sensory organ that requires mechanosensitive hair cells to detect linear acceleration. In neonatal mice, new hair cells are derived from non-sensory supporting cells, yet cell type diversity and mechanisms of cell addition remain poorly characterized.
Simon Cross et al.
Journal of chemical information and modeling, 52(10), 2587-2598 (2012-09-14)
Pharmacophore elucidation approaches are routinely used in drug discovery, primarily with the aim of determining the three-dimensional arrangement of common features shared by ligands interacting at the site of interest; these features can then be used to investigate the structure-activity
Raymond G Sierra et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 11), 1584-1587 (2012-10-24)
An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min(-1) and diffracted to beyond
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