T7924

Sigma-Aldrich

Transforming Growth Factor-α human

TGF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

Synonym(s):
hTGF-α, TGF-α
Empirical Formula (Hill Notation):
C239H348N70O72S6
CAS Number:
Molecular Weight:
5546.14
MDL number:
NACRES:
NA.77

Quality Level

biological source

human

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE and HPLC)

form

powder

potency

≤0.2 ng/mL ED50

quality

endotoxin tested

mol wt

5.5 kDa

packaging

pkg of 1 mg
pkg of 5 mg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... TGFA(7039)

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Amino Acid Sequence

Val-Val-Ser-His-Phe-Asn-Asp-Cys-Pro-Asp-Ser-His-Thr-Gln-Phe-Cys-Phe-His-Gly-Thr-Cys-Arg-Phe-Leu-Val-Gln-Glu-Asp-Lys-Pro-Ala-Cys-Val-Cys-His-Ser-Gly-Tyr-Val-Gly-Ala-Arg-Cys-Glu-His-Ala-Asp-Leu-Leu-Ala [Disulfide bridges: 8-21; 16-32; 34-43]

General description

The TNFα (tumor necrosis factor α) gene is mapped to human chromosome 6p21.33. TNFα is a member of TNF superfamily.

Application

Transforming Growth Factor-α human has been used:
  • to determine the ideal concentration of growth factors to be used in the culture medium that supports sheep preantral follicles
  • in human hepatocytes cell culture and keratinocyte cell culture
  • to induce morphogenesis in primary mouse organoids

Biochem/physiol Actions

The TNFA gene is significantly associated with the regulation of innate and adaptive immune responses. Polymorphism of TNFA gene is known to cause acute motor axonal neuropathy. The cytokine release and gene action is associated with epithelial cells, myeloid cells, endothelial, as well as tumor cells. Tumor cells are known to release TNFA, specifically in response to chemotherapy.
Transforming growth factor-α (TGF-α), is a 5.5 kDa polypeptide containing 50 amino acids in its mature form. It was originally identified as an agent that reversibly confers a transformed phenotype upon normal non-neoplastic cells, such as normal rat kidney fibroblasts. This activity requires the presence of transforming growth factor-β (TGF-β), which potentiates the action of TGF-α via a separate receptor. TGF-α is synthesized by monocytes, keratinocytes and many tissues and tumors. Mice genetically engineered to be lacking TGF-α expression showed no abnormalities except for the detection of a "wavy" hair coat. TGF-α exerts its action through the EGF receptor.

Physical form

Lyophilized from a 0.2 μm filtered buffered solution.

Analysis Note

The biological activity is measured by its ability to stimulate 3H-thymidine incorpation into BALB/c 3T3 cells.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Nuclear maturation of ovine oocytes in cultured preantral follicles
Tamilmani G, et al.
Small Ruminant Research, 60(3), 295-305 (2005)
Srcasm modulates EGF and Src-kinase signaling in keratinocytes
Li W, et al.
The Journal of Biological Chemistry, 280(7) (2005)
LSHGD: A database for human leprosy susceptible genes
Doss CGP, et al.
Genomics, 100(3), 162-166 (2012)
Tsuyoshi Takagi et al.
Endocrinology, 148(6), 2788-2792 (2007-03-17)
The molecular mechanism underlying photoperiodism is not well understood in any organism. Long-day-induced conversion of prohormone T(4) to bioactive T(3) within the mediobasal hypothalamus (MBH) is critical for the photoperiodic regulation of reproduction. However, because thyroidectomy does not completely block...
Inflammatory cytokine production in tumor cells upon chemotherapy drug exposure or upon selection for drug resistance
Edwardson DW, et al.
PLoS ONE, 12(9), e0183662-e0183662 (2017)

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