T8443
Trizma® Pre-set crystals
BioPerformance Certified, pH 8.0, average Mw 141.8
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grade
BioPerformance Certified
form
crystalline powder
mol wt
average Mw 141.8
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, NICKase, none detected
endotoxin, tested
total aerobic microbial count, tested
≤0.05 A290
≤0.5% water (Karl Fischer)
≤5 ppm Heavy metals (as Pb)
pH
8.00 (25 °C, 0.1 M)(lit.)
8.0
useful pH range
7.0-9.0
solubility
water: 0.66 g/mL, clear, colorless
application(s)
diagnostic assay manufacturing
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This Item | T9943 | T9693 | T8568 |
|---|---|---|---|
| grade BioPerformance Certified | grade BioPerformance Certified | grade BioPerformance Certified | grade BioPerformance Certified |
| form crystalline powder | form crystalline powder | form crystalline powder | form crystalline powder |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| impurities DNase, RNase, Protease, NICKase, none detected, endotoxin, tested, total aerobic microbial count, tested, ≤0.05 A290, ≤0.5% water (Karl Fischer), ≤5 ppm Heavy metals (as Pb) | impurities DNase, RNase, Protease, NICKase, none detected, endotoxin, tested, total aerobic microbial count, tested, ≤0.05 A290, ≤0.5% water (Karl Fischer), ≤5 ppm Heavy metals (as Pb) | impurities DNase, RNase, Protease, NICKase, none detected, endotoxin, tested, total aerobic microbial count, tested, ≤0.05 A290, ≤0.5% water (Karl Fischer), ≤5 ppm Heavy metals (as Pb) | impurities DNase, RNase, Protease, NICKase, none detected, endotoxin, tested, total aerobic microbial count, tested, ≤0.05 A290, ≤0.5% water (Karl Fischer), ≤5 ppm Heavy metals (as Pb) |
| pH 8.00 (25 °C, 0.1 M)(lit.) | pH 6.70 (37 °C, 0.1 M)(lit.), 7.00 (25 °C, 0.1 M)(lit.), 7.55 (5 °C, 0.1 M)(lit.) | pH 9.00 (25 °C, 0.1 M)(lit.) | pH 8.10 (25 °C, 0.1 M)(lit.) |
| solubility water: 0.66 g/mL, clear, colorless | solubility - | solubility water: 0.66 g/mL, clear, colorless | solubility water: 0.666 g/mL, clear, colorless |
Application
Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.
If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
Other Notes
Preparation Note
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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