T8690

Thioredoxin human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg

• CAS Number: 52500-60-4
• MDL number: MFCD00132423
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli (N-terminal histidine tagged)
• assay: ≥90% (SDS-PAGE)
• form: essentially salt-free, lyophilized powder
• specific activity: ≥5 U/mg
• mol wt: 14 kDa
• technique(s): activity assay: suitable
• solubility: water: soluble 0.50—0.60 mg/mL, clear to slightly hazy, colorless to faint yellow or tan
• suitability: suitable for molecular biology
• UniProt accession no.: P10599
• application(s): cell analysis
• storage temp.: −20°C
• Gene Information: human ... TXN(7295)

Description

General description

Cloned from Jurkat cell cDNA. Identical to the ADF/Trx (GenBank^® Accession Number X77584) and the thioredoxin from human placenta (GenBank^® Accession Number AF085844).

Thioredoxin (TXN) is a 12 kDa ubiquitous protein. The gene is located on human chromosome 9q31.3. TXN is mainly located in the cytoplasm.[1]Thioredoxin contains a redox-active dithiol in the active site. Itexists in several forms, the most prevalent ones being the cytosolic (TRX1) andmitochondrial (TRX2) forms.[2]

Application

Thioredoxin human can be used for in vitro thioredoxin assay.[3] The product can also be used for assessing Thioredoxin reductase (TRXR) enzyme activity.[4]

Thioredoxin human has been used:

• in mitogen-activated protein kinase kinase 4 (MKK4) in vitro kinase assay[5]
• to study its role in transcription regulation, RNA processing and nuclear pore function in human cells[6]
• in proteomics identification of Trx1 reduction target proteins from the hearts of cardiac specific transgenic (Tg)-Trx1 mice[7]

Biochem/physiol Actions

Human Thioredoxin is a recombinant, N-terminal histidine tagged protein. The product induces proliferation in lymphoid cells, fibroblasts as well as in numerous human solid tumor cell lines.[8]The product also stimulates growth in normal as well as leukemic B cell,[9] modifies the transcriptional activity of AP-1[10]and inhibits the apoptosis signal-regulating kinase 1 (ASK1).[11]Thioredoxin (TRX) plays a role in protecting the cells fromelectrophiles, a cytotoxic agent.[2]

Mediates the reduction of disulfide bonds in proteins.

Thioredoxin (TXN) has disulfide-reducing activity. It acts as a cytokine to reduce inflammation. TXN functions as a scavenger for reactive oxygen intermediates (ROIs), which are produced in various oxidative stress. Thioredoxin induces cellular proliferation to decrease oxidative stress. TXN is a prognostic biomarker for various cancers, such as lung, pancreatic and hepatocellular carcinoma.[1] It contributes to protein reduction, tissue development and redox regulation.[5]

Unit Definition

One unit will cause a ΔA₆₅₀ of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)