T8768

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate

horseradish peroxidase substrate, chromogenic, ≥98%, powder

• Synonym(s): TMB dihydrochloride
• Linear Formula: [-C₆H₂(CH₃)₂-4-NH₂]₂·2HCl·xH₂O
• CAS Number: 207738-08-7
• Molecular Weight: 313.27 (anhydrous basis)
• MDL number: MFCD00150104
• UNSPSC Code: 12352204
• PubChem Substance ID: 24900558
• NACRES: NA.83

Properties

• Product Name: 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate, powder, ≥98%
• assay: ≥98%
• form: powder
• solubility: acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy
• storage temp.: 2-8°C
• SMILES string: O.Cl.Cl.Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
• InChI: 1S/C16H20N2.2ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;/h5-8H,17-18H2,1-4H3;2*1H;1H2
• InChI key: KVCWTKDFVVSVSJ-UHFFFAOYSA-N

Description

General description

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase.[1] It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.[2]

Application

3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay[3] and reverse dot blot (RDB).[4]

Noncarcinogenic substitute for benzidine.

Safety Information

• pictograms: GHS08,GHS07
• signalword: Warning
• hcodes: H302,H351,H413
• pcodes: P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)