T8823

Monoclonal Anti-T7 tag antibody produced in mouse

purified immunoglobulin, buffered aqueous solution

• Synonym(s): T7 Tag antibody
• MDL number: MFCD02689159
• UNSPSC Code: 12352203
• NACRES: NA.56

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: T7 Tag
• form: buffered aqueous solution
• concentration: ~2 mg/mL
• technique(s): western blot (chemiluminescent): 1-2 μg/mL using recombinant T7-tagged β-galactosidase
• isotype: IgM
• shipped in: dry ice
• Storage temp.: −20°C

Description

General description

The Mouse monoclonal Anti-T7 tag antibody recognizes the T7 tag sequence on T7-tagged fusion proteins. The T7 tag functions as a marker in many expression vectors.

The T7 tag is a 11 amino acid peptide,[1] encoded in the leader sequence of T7 bacteriophage gene 10.[2] This gene encodes a T7 major capsid protein.[3]

Immunogen

synthetic peptide corresponding to the T7-tag (MASMTGGQQMG-K), conjugated to KLH

Application

Monoclonal Anti-T7 tag antibody produced in mouse has been used in western blotting.[4]

Physical form

Solution at approximately 2 mg/ml in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Analysis Note

Antibody is suitable for ELISA and immunoblotting.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable