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T9026

Sigma-Aldrich

Monoclonal Anti-α-Tubulin antibody produced in mouse

enhanced validation

clone DM1A, ascites fluid

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Synonym(s):
Monoclonal Anti-α-Tubulin
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

DM1A, monoclonal

mol wt

antigen ~50 kDa

contains

15 mM sodium azide

species reactivity

yeast, mouse, amphibian, human, rat, chicken, fungi, bovine

enhanced validation

independent ( antibodies)
Learn more about Antibody Enhanced Validation

technique(s)

indirect immunofluorescence: 1:500 using cultured chicken fibroblasts
western blot: 1:500 using human or chicken fibroblasts

isotype

IgG1

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

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This Item
F2168T6199T7451
antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

FITC conjugate

conjugate

unconjugated

conjugate

unconjugated

clone

DM1A, monoclonal

clone

DM1A, monoclonal

clone

DM1A, monoclonal

clone

6-11B-1, monoclonal

species reactivity

yeast, mouse, amphibian, human, rat, chicken, fungi, bovine

species reactivity

yeast, mouse, amphibian, human, rat, chicken, fungi, bovine

species reactivity

yeast, mouse, amphibian, human, rat, chicken, fungi, bovine

species reactivity

bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant

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General description

α-tubulin, also called tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family, and contains 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC. Anti-α-Tubulin FITC antibody, Mouse monoclonal specifically recognizes an epitope in the carboxy terminal part of α-tubulin. It localizes α-tubulin in human, monkey, bovine, chicken, goat, murine, rat, gerbil, hamster, rat kangaroo, amphibia, sea urchin, trypanosome, yeast, fungi and tobacco.

Specificity

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.

Immunogen

microtubules from chicken embryo brain.

Application

Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in western blot analysis and protein expression analysis.
Monoclonal Anti-α-Tubulin antibody produced in mouse has been used in the detection of α-Tubulin:
  • in human osteosarcoma and in breast cancer cell lines by western blotting
  • in HeLa cells by immunofluorescence microscopy
  • by immunostaining
  • immunohistochemical detection in Xenopus embryos

Biochem/physiol Actions

Tubulin α 4a (TUBA4A) mutation is associated with amyotrophic lateral sclerosis (ALS).
α/β-Tubulin is the major building block of microtubules. These intracellular, hollow, cylindrical, filamentous structures are present in virtually all eukaryotic cells. Self-assembly of α/β-tubulin leads to polar microtubular structures built from linearly arranged strings of alternatively α- and β-tubulin pairs pointing in the same direction. Microtubules function as structural and mobile elements in mitosis, intracellular transport, ciliary flagellar motility and generation and maintenance of cell shape. α/β-Tubulin and γ-tubulin are members of the tubulin superfamily of proteins. α/β-Tubulin is a heterodimer which consists of one α-tubulin chain and one β-tubulin chain; each subunit has a molecular weight of 55 kDa and they share considerable homolog.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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TRAIL induces apoptosis in triple-negative breast cancer cells with a mesenchymal phenotype.
Rahman M, et al.
Breast Cancer Research and Treatment, 113(2), 217-230 (2009)
CHIP overexpression reduces mutant androgen receptor protein and ameliorates phenotypes of the spinal and bulbar muscular atrophy transgenic mouse model.
Adachi H
The Journal of Neuroscience, 27(19), 5115-5126 (2007)
Telomere maintenance through spatial control of telomeric proteins.
Chen LY
Molecular and Cellular Biology, 27(16), 5898-5909 (2007)
MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization.
Suzuki M, et al.
Development, dev-048769 (2010)
Analysis of gene function in somatic mammalian cells using small interfering RNAs.
Elbashir SM, et al.
Methods, 26(2), 199-213 (2002)

Articles

Uses for immunofluorescence (IF)—where an antibody conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-translational modification or activation, and proximity to/complexing with other proteins.

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.

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