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T9157

Sigma-Aldrich

Tryptose Phosphate Broth

suitable for insect cell culture

biological source

bovine milk

Quality Level

form

solid

technique(s)

cell culture | insect: suitable

shipped in

ambient

storage temp.

room temp

General description

Tryptose Phosphate Broth (TPB) comprises pyrimidine based components and is crucial for pyrimidine synthesis and electron transport chain in cells. In chick embryo fibroblast, it alleviates chloramphenicol inhibition.

Application

In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
Tryptose Phosphate Broth has been used as a supplement:
  • in Eagle′s minimum essential medium alpha for growing Vero cells
  • for Vero-CCL81 cells in porcine epidemic diarrhea coronavirus propagation
  • in Dulbecco modified Eagle medium in LR7cells for mouse hepatitis virus propagation
  • in Glasgow minimum essential medium (GMEM) for Madin-Darby canine kidney (MDCK) cells.

Packaging

100, 500 g in poly bottle

Components

Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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