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T9179
Terrific Broth
EZMix powder microbial growth medium
Synonym(s):
TB media
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| Size/SKU | Availability | Price |
|---|---|---|
6 x 500 mL | Check Cart for Availability | $85.30 |
2 x 5 L | Check Cart for Availability | $203.00 |
1 kg | Check Cart for Availability | $324.00 |
About This Item
NACRES:
NA.85
UNSPSC Code:
41106200
$85.30
Check Cart for Availability
grade
Molecular Biology
Quality Segment
description
TB; quick dissolve, dust-free formulation
sterility
non-sterile
form
powder
technique(s)
microbiological culture: suitable
application(s)
agriculture
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.
Application
Suitable for non-selective cultivation of E. coli strains for cloning, high yield DNA plasmid production and high yield production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Terrific broth has been used:
Terrific broth has been used:
- as a medium for selective growth of E.coli BL21 (DE3) V2RpRARE expressing MLL SET (nuclear proto-oncogene) domain in pET28GST-LIC
- to grow BL21 (DE3) cells containing plasmid expressing IL-8 (interleukin-8)
- Terrific broth is also suitable for use in bacterial suspension (Bacillus subtilis strain 1085B)
Features and Benefits
Terrific Broth EZMix powder provides:
- Granulated, dust-free format for safer handling and faster mixing
- A budget-friendly alternative to liquid
- Modified formulation (without glycerol)
- Easy scale-up using larger package sizes
Preparation Note
Suspend 24.1 g of powder and 4 mL of glycerol in sufficient deionized water to make 500 mL. Autoclave for 15 minutes at 121 °C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Other Notes
*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description EZMix powder microbial growth medium | description Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture | description Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture. | description Liquid microbial growth medium |
| form powder | form powder | form powder | form liquid |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for coliforms, nonselective for Escherichia coli | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for coliforms, nonselective for Escherichia coli |
| technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility sterile; 0.2 μm filtered |
| application(s) agriculture | application(s) food and beverages | application(s) food and beverages | application(s) microbiology |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the Department of Transportation shipping information for this product?
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Which bacterial culture medium is the best choice for my application?
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Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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