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MilliporeSigma

T9281

Sigma-Aldrich

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, suitable for electrophoresis, ≥99.0%

N,N,N′,N′-Tetramethylethylenediamine

Synonym(s):

1,2-Bis(dimethylamino)ethane, TEMED, TMEDA

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50 ML
$99.90
100 ML
$190.00

About This Item

Linear Formula:
(CH3)2NCH2CH2N(CH3)2
CAS Number:
Molecular Weight:
116.20
Beilstein/REAXYS Number:
1732991
EC Number:
MDL number:
UNSPSC Code:
41105319
PubChem Substance ID:
NACRES:
NB.22

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$99.90


In StockDetails


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product line

BioReagent

assay

≥99.0%

expl. lim.

9.08 %

technique(s)

electrophoresis: suitable

refractive index

n20/D 1.4179 (lit.)

bp

120-122 °C (lit.)

mp

−55 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

suitability

suitable for electrophoresis

SMILES string

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

InChI key

KWYHDKDOAIKMQN-UHFFFAOYSA-N

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This Item
411019T7024T22500
assay

≥99.0%

assay

≥99.5%

assay

≥99% (GC)

assay

99%

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

mp

−55 °C (lit.)

mp

−55 °C (lit.)

mp

−55 °C (lit.)

mp

−55 °C (lit.)

refractive index

n20/D 1.4179 (lit.)

refractive index

n20/D 1.4179 (lit.)

refractive index

n20/D 1.4179 (lit.)

refractive index

n20/D 1.4179 (lit.)

density

0.775 g/mL at 20 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

density

0.775 g/mL at 20 °C (lit.)

bp

120-122 °C (lit.)

bp

120-122 °C (lit.)

bp

120-122 °C (lit.)

bp

120-122 °C (lit.)

General description

TEMED (N,N,N′,N′-Tetramethylethylenediamine) is molecule which allows rapid polymerization of polyacrylamide gels. In the presence of ammonium persulfate, TEMED is responsible for the formation of free radicals from persulfate, thereby initiating the acrylamide polymerization process. However, excess concentration of TEMED can result in abnormal separation patterns.[1]

Application

TEMED (N,N,N′,N′-Tetramethylethylenediamine) has been used for the preparation of acrylamide hydrogels and sodium dodecyl sulfate-polyacrylamide gels.[2][3][4]

Caution

Handle and store under inert gas, air and moisture sensitive.

Analysis Note

Tested as a catalyst for PAGE.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

61.7 °F - closed cup

flash_point_c

16.5 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


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Questions

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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