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T9283

Sigma-Aldrich

Monoclonal Anti-Tropomyosin (Sarcomeric) antibody produced in mouse

clone CH1, ascites fluid

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Synonym(s):
Anti-C15orf13, Anti-CMD1Y, Anti-CMH3, Anti-HEL-S-265, Anti-HTM-alpha, Anti-LVNC9, Anti-TMSA
MDL number:

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

CH1, monoclonal

contains

15 mM sodium azide

species reactivity

human, chicken, rat

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using sections of human tongue
immunohistochemistry (frozen sections): suitable using human and animal tissue sections
immunoprecipitation (IP): suitable
microarray: suitable
western blot: suitable using native preparations

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

chicken ... TPM1(396366)
human ... TPM1(7168)
rat ... Tpm1(24851)

General description

Tropomyosin 1 (TPM1) is encoded by the gene mapped to human chromosome 15q22.2. The encoded protein belongs to the TPM protein family.

Specificity

The antibody recognizes an epitope on skeletal and cardiac tropomyosin. The antibody shows no reaction with denatured protein by immunoblotting. No cross-reactivity has been observed with smooth muscle or non-muscle tropomyosin.

Immunogen

chicken muscle tropomyosin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Monoclonal Anti-Tropomyosin (sarcomeric) antibody produced in mouse has been used in immunofluorescence and immunohistochemistry.

Biochem/physiol Actions

Tropomyosin 1 (TPM1) is a potent tumor suppressor gene. It is expressed at low levels in various types of solid cancers including, oral squamous cell carcinoma (OSCC) and tongue squamous cell carcinoma. Mutation in the gene is associated with the development of hypertrophic cardiomyopathy. The encoded protein is involved in the regulation of actin-myosin interaction in striated muscles. Tropomyosin plays an essential role in Ca2+ -regulated contraction of cardiac muscle.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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