T9285

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

• Synonym(s): TE buffer solution
• MDL number: MFCD00236359
• PubChem Substance ID: 329826904
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• sterility: 0.2 μm filtered
• form: liquid
• pH: 7.8-8.2 (1 × in water)
• foreign activity: DNase, RNase, none detected
• storage temp.: room temp
• SMILES string: NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
• InChI key: VLEIUWBSEKKKFX-UHFFFAOYSA-N

Description

General description

TE buffer is the most commonly used buffer solution in molecular biology. "TE" is a component of Tris (a common pH buffer) and EDTA (molecule that chelate cations). TE (Tris EDTA) Buffer solubilizes DNA and RNA while protecting it from degradation. TE Buffer is used in nucleic acid isolation, which may be done prior to Northern or Southern blot hybridization.

Application

Suitable for storage and isolation of RNA and DNA, including purified plasmid DNA stocks.

• Tris-EDTA buffer solution has been used in culture medium.[1][2]
• Tris-EDTA buffer solution has been used for the preparation of AmpC disks for permeabilization of Tris-EDTA to release β-lactamases from bacterial cell.[3]
• It has been used for the suspension of bacterial spores.[4]
• It has also been used as a buffer in immunohistochemistry.[5]

Components

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable