MilliporeSigma
All Photos(3)

Documents

T9285

Sigma-Aldrich

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

Sign Into View Organizational & Contract Pricing

Synonym(s):
Tris-EDTA wash buffer, TE buffer solution
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

sterility

0.2 μm filtered

form

liquid

pH

7.8-8.2 (1 × in water)

application(s)

sample preparation

foreign activity

DNase, RNase, none detected

storage temp.

room temp

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
93283T4415T9650
Tris-EDTA buffer solution 100X concentrate for Northern and Southern blotting

T9285

Tris-EDTA buffer solution

-
Tris-EDTA buffer solution BioUltra, for molecular biology, pH 8.0

93283

Tris-EDTA buffer solution

Premium Grade
Tris-Borate-EDTA buffer BioReagent, suitable for electrophoresis, 10× concentrate

T4415

Tris-Borate-EDTA buffer

Essential+ Grade
Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

T9650

Tris Acetate-EDTA buffer

Essential+ Grade
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

application(s)

sample preparation

application(s)

sample preparation

application(s)

-

application(s)

-

form

liquid

form

liquid

form

solution

form

solution

pH

7.8-8.2 (1 × in water)

pH

8.0±0.2 (25 °C)

pH

-

pH

-

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

General description

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

application

TE buffer has been used for:
  • isolate, resuspend, or store DNA and RNA for PCR or qPCR
  • gel electrophoresis, including Northern and Southern blotting
  • bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
  • antigen retrieval for immunohistochemistry

Components

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Brian O'Farrell et al.
PloS one, 7(10), e48022-e48022 (2012-11-13)
Microbial genotyping increasingly deals with large numbers of samples, and data are commonly evaluated by unstructured approaches, such as spread-sheets. The efficiency, reliability and throughput of genotyping would benefit from the automation of manual manipulations within the context of sophisticated
Pak Kin Wong et al.
Analytical chemistry, 76(23), 6908-6914 (2004-12-02)
Bioprocessors for concentrating bioparticles, such as cells and molecules, are commonly needed in bioanalysis systems. In this microfluidic processor, a global flow field generated by ac electroosmosis transports the embedded particles to the regions near the electrode surface. The processor
Emma Lorenzen et al.
Frontiers in immunology, 6, 628-628 (2016-01-07)
International efforts in developing a vaccine against Chlamydia trachomatis have highlighted the need for novel immunization strategies for the induction of genital immunity. In this study, we evaluated an intramuscular (IM) prime/intranasal boost vaccination strategy in a Göttingen Minipig model
Shiraz Tyebji et al.
Cell reports, 31(4), 107573-107573 (2020-04-30)
Germline epigenetic factors influence transgenerational inheritance of behavioral traits upon changes in experience and environment. Immune activation due to infection can also modulate brain function, but whether this experience can be passed down to offspring remains unknown. Here, we show
AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases.
Black JA, et al.
Journal of Clinical Microbiology, 43, 3110-3113 (2005)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service