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T9455

Sigma-Aldrich

TMB Enhanced One Component HRP Membrane Substrate

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Synonym(s):
HRP substrate, TMB, detection reagent, detection substrate
UNSPSC Code:
12352204
NACRES:
NA.83

form

solution

Quality Level

storage temp.

2-8°C

General description

TMB Enhanced One Component HRP Membrane Substrate is a ready-to-use solution containing 3,3′,5,5′-tetramethylbenzidine (TMB) in a mildly acidic buffer. TMB is a substrate for horseradish peroxidase. The substrate system will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. This substrate is not recommended for microwell or immunohistochemical applications.

Application

TMB Enhanced One Component HRP Membrane Substrate has been used as a colorimetric substrate for horseradish peroxidase (HRP) in an electrochemical enzymatic sandwich assay for the detection of interleukin-6 (IL-6) in biological fluids.

Caution

Stable at least one year at 2-8 °C.

Physical form

Ready-to-use.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Protocols

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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