Trizma® Pre-set crystals

BioPerformance Certified, pH 9.0, average Mw 124.6

Tris crystals

Quality Level


BioPerformance Certified


crystalline powder

mol wt

average Mw 124.6


cell culture | mammalian: suitable


DNase, RNase, Protease, NICKase, none detected
endotoxin, tested
total aerobic microbial count, tested
≤0.05 A290
≤0.5% water (Karl Fischer)
≤5 ppm Heavy metals (as Pb)


8.70 (37 °C, 0.1 M)(lit.)
9.00 (25 °C, 0.1 M)(lit.)
9.56 (5 °C, 0.1 M)(lit.)

useful pH range

7.0 - 9.0


water: 0.66 g/mL, clear, colorless

Featured Industry

Diagnostic Assay Manufacturing

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Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.
The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.


100, 250 g in poly bottle

Other Notes

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.
Certain pH electrodes do not give accurate results with Tris buffers.

Preparation Note

Trizma solutions can be autoclaved.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

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