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T9777

Sigma-Aldrich

Trypsin-chymotrypsin inhibitor from Glycine max (soybean)

lyophilized powder

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Synonym(s):
Bowman-Birk Inhibitor
CAS Number:
MDL number:
NACRES:
NA.77

biological source

Glycine max (soybean)

Quality Level

form

lyophilized powder

composition

Protein, 70-90% biuret

storage temp.

2-8°C

Related Categories

General description

Trypsin-chymotrypsin inhibitor belongs to the family of plant protease inhibitors. It is mainly found in the legume (Fabaceae) and cereal (Poaceae) families. Trypsin-chymotrypsin inhibitor consists of many internal disulfide bonds between conserved Cys residues, which maintains structural stability and active conformation of the inhibitory loops. Trypsin-chymotrypsin has anticarcinogenic properties.
Highly cross-linked with seven disulfide bridges, Bowman-Birk inhibitor has spatially distinct domains for trypsin and chymotrypsin inhibition.

Application

Bowman Birk protease inhibitor prevents radiation-induced carcinogenesis by a reduction of incorrect DNA repairs, resulting in a reduced amount of dicentric chromosomes.
Trypsin-chymotrypsin inhibitor from Glycine max (soybean) has been used for flow cytometric separase activity assay. It has also been used to inhibit trypsin and chymotrypsin for in vitro gastrointestinal (GI) digestion studies.

Unit Definition

One trypsin unit = ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C. Reaction volume = 3.2 mL (1 cm light path).

Physical form

Lyophilized powder containing phosphate buffer salts, pH 7.6

Analysis Note

1 mg protein will inhibit ≥0.5 mg trypsin with activity of ~10,000 BAEE units per mg protein or ≥1.0 mg chymotrypsin with activity of ~40 BTEE units per mg protein.

Other Notes

View more information on Trypsin Inhibitor.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Articles

Analytical Enzyme Chymotrypsin: Chymotrypsin is produced in the acinar cells of the pancreas as the inactive precursor, chymotrypsinogen.

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