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TOX1

Sigma-Aldrich

In Vitro Toxicology Assay Kit, MTT based

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Synonym(s):
mitochondrial activity assay
NACRES:
NA.84

usage

 kit sufficient for 1,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

λmax

570 nm

application(s)

cell analysis
detection

detection method

colorimetric

storage temp.

2-8°C

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TOX7TOX6CT01
vibrant-m

CT01

MTT Cell Growth Assay Kit

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

-

storage condition

dry at room temperature

storage condition

dry at room temperature

storage condition

dry at room temperature

storage condition

-

λmax

570 nm

λmax

490 nm

λmax

565 nm

λmax

-

cytotoxicity assay substrate, MTT dye based, colorimetric determination, 570 nM detection

cytotoxicity assay substrate, tetrazolium dye based, colorimetric determination, 490 nM detection

cytotoxicity assay substrate, colorimetric determination, 565 nM detection

-

application(s)

cell analysis
detection

application(s)

cell analysis
detection

application(s)

cell analysis
detection

application(s)

-

General description

This kit is designed for determining cell number/ cell viability spectrophotometrically as a function of mitochondrial activity in living cells. The MTT method is simple, accurate and yields reproducible results. The key component is (3- [4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) or MTT. Solutions of MTT, dissolved in medium or balanced salt solutions without phenol red, are yellowish in color. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple formazan crystals which are insoluble in aqueous solutions. Absorbance of converted dye is measured at a wavelength of 570nm.

Application

In Vitro Toxicology Assay Kit, MTT based has been used to perform an (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay to measure the cytotoxicity of gold nanoparticles (AuNP). It has also been used to examine cell viability in SH-SY5Y cultures in response to 6-hydroxydopamine (6-OHDA) treatment.

Biochem/physiol Actions

Conversion of MTT to a water-insoluble colored formazan derivative which is then solubilized in acidic isopropanol.

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

53.6 °F

flash_point_c

12 °C


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Articles

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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