This kit utilizes Neutral Red, a vital dye, to measure cell viability after exposure to any specific potential toxin. Neutral red is only taken up by viable cells. Cell viability is determined by measuring the absorbance following the specified incubation period to the potential toxin of interest. A positive control is not included and would be the untreated cells. In this case, the viability of cells exposed to a given wavelength of light compared to cells that have not been exposed. Please see the link below to review the technical bulletin for this kit:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/114/325/tox4bul.pdf
TOX4
In Vitro Toxicology Assay Kit, Neutral Red based
Synonym(s):
NR uptake assay, Neutral red assay
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.84
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$370.00
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form
liquid
usage
kit sufficient for 1,000 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
λmax
540 nm
application(s)
cell analysis
detection
detection method
colorimetric
storage temp.
2-8°C
1 of 4
This Item | TOX8 | TOX1 | TOX7 |
|---|---|---|---|
| form liquid | form liquid | form - | form liquid |
| usage 1 kit sufficient for 1,000 tests | usage 1 kit sufficient for 2,000 tests | usage 1 kit sufficient for 1,000 tests | usage kit sufficient for 500 tests |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit |
| storage condition dry at room temperature | storage condition dry at room temperature | storage condition dry at room temperature | storage condition dry at room temperature |
| λmax 540 nm | λmax - | λmax 570 nm | λmax 490 nm |
cytotoxicity assay substrate, colorimetric determination, 540 nM detection | cytotoxicity assay substrate, tetrazolium dye based, fluroescence determination, 600 nM detection | cytotoxicity assay substrate, MTT dye based, colorimetric determination, 570 nM detection | cytotoxicity assay substrate, tetrazolium dye based, colorimetric determination, 490 nM detection |
General description
For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm.
Application
Biochem/physiol Actions
Neutral red (NR) is a red dye that accumulates in the lysosome by actively transferring across the cell membranes.[3] The dye is extracted and the uptake is quantitated by spectroscopy. The NR uptake assay is the most commonly used cytotoxicity test. It is widely applicable in environmental and biomedical studies to estimate the number of viable cells in culture. The NR dye in vitro tissue culture studies is used in assessing viral cytopathogenicity and detection of toxic compounds.[4]
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Flam. Liq. 3 - Skin Sens. 1
Storage Class
3 - Flammable liquids
wgk_germany
WGK 3
flash_point_f
73.4 °F
flash_point_c
23 °C
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Can I use this kit for phototoxicity assay. If so, does the kit contain a positive control for phototoxicity?
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