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MilliporeSigma

TP0300

Sigma-Aldrich

Total Protein Kit, Micro Lowry, Peterson′s Modification

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1 KIT
$255.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

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$255.00


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usage

sufficient for ~200 assays

Quality Level

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This Item
PE0230MOD50L3540
usage

sufficient for ~200 assays

usage

-

usage

sufficient for 50 reactions

usage

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

General description

This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.

Application

Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.[1][2][3]

Features and Benefits

  • Complete kit for protein assays
  • Most widely cited protein assay
  • Less protein to protein variation than other assays
  • Contains reagents that can remove interfering substances

Principle

An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

wgk_germany

WGK 3


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Questions

  1. How many tests can one run with this kit?

    1 answer
    1. As listed in the 'PROPERTIES' section, this kit is sufficient for approximately 200 tests. Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/184/250/tp0300bul.pdf

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